石蝉草内生细菌多样性及其特征  

Diversity and Characteristics of Endophytic Bacteria Isolated from Peperomia dindygulensis Miq

作　　者：靳松[1,2] 陈泽斌[1] 李育川[1] 夏体渊[1] 任禛[1] 莫丽玲[1] 华金珠[1] 

机构地区：[1]昆明学院农学院,云南昆明650214 [2]云南省高校特色生物资源开发与利用重点实验室,云南昆明650214

出　　处：《福建农业学报》2016年第10期1104-1108,共5页Fujian Journal of Agricultural Sciences

基　　金：云南省教育厅科学研究基金项目(2015Y393);昆明学院科学研究项目(XJZD1605);昆明学院大学生科研项目(XJD16082);昆明学院人才引进项目(YJL14005)

摘　　要：采用菌落表征性状统计和16SrRNA基因序列分析法,对石蝉草根、茎、叶内生细菌的数量分布、群落组成和遗传多样性进行初步研究。从各组织器官中分离出内生细菌383株,通过菌落表征性状,以类平均连锁聚类法进行聚类分析,在欧氏距离为0的水平上可聚为19个亚群。多样性分析结果表明,19个亚群隶属于8属13种;其数量表现为叶>根>茎,多样性表现为茎>叶>根,其中鞘氨醇单胞菌属Sphingomonas和寡养单胞菌属Stenotrophomonas为石蝉草内生细菌的优势属。Statistical classification of colony characteristics and 16S rRNA gene sequencing were performed to obtain the information on the quantitative distribution, community composition, and diversity of endophytic bacteria in the roots, stems and leaves of Peperomia dindygulensis Miq. A total of 383 endophytic bacteria were isolated and clustered according to their colony characteristics. After the average linkage clustering, the groups were divided into 19 subgroups at the Euclidean distance of 0 level. The subgroups were phylogenetically classified into 8 genera and 13 species based on their partial 16S rDNA sequences. The quantities of endophytic bacteria in the tissues were in order of leaves〉 roots〉 stems; and, the diversity of the bacteria, stems〉leaves〉roots. The predominant endophytic bacteria in P. dindygulensis Miq were found to belong to the genera of Sphingomonas and Stenotrophomonas.

关 键 词：石蝉草 内生细菌 多样性 16S RRNA 

分 类 号：Q939.96[生物学—微生物学]