^(60)Co-γ射线辐照翠竹的生物学效应及RAPD分析  

Biological Effects and RAPD Analysis of ^(60)Co-γ-radiation on Sasapygmaea

作　　者：赵婧妤[1] 贺诗雨 戴刘凤 翟敬宇 周云龙[1] 谭锬[1,3] 梁前进[1] 

机构地区：[1]北京师范大学生命科学学院,北京100875 [2]北京市紫竹院公园管理处,北京100048 [3]南华大学药学与生物科学学院/湖南省分子靶标新药研究协同创新中心,衡阳421001

出　　处：《世界科技研究与发展》2016年第6期1280-1286,共7页World Sci-Tech R&D

基　　金：北京师范大学本科生科研训练与创新创业基金(201605)资助

摘　　要：用不同剂量(5、10、15、20和25 Gy)的^(60)Co-γ射线对翠竹幼苗进行辐照处理,结果显示,辐照导致翠竹矮化,植株的株高、节间长度、叶长及叶宽等性状较对照组显著变短;并且10 Gy辐照导致翠竹部分叶片白化。基因组RAPD分子标记检测显示,辐照组扩增的DNA片段图谱发生显著变化,表明经辐照处理的翠竹基因组发生了变异。其中,5 Gy辐照实验组的变异程度最小,25 Gy辐照实验组的变异程度最大。研究表明,^(60)Co-γ射线辐照可以引起翠竹植株矮化,并导致基因组变异。本研究为进一步进行翠竹辐照诱变育种打下了基础。Various radiation dosages（5 Gy,10 Gy,15 Gy,20 Gy and 25 Gy）of ^60Co-γ-radiation are used to treat the seed-lings of Sasapygmaea. The results show that irradiation can induce dwarf effect, and the plant height, internode length, leaf length and width of irradiation group are significantly shorter than the control group. The leaves of Sasapygmaea in 10 Gy ra- diation dosage group show albinism. By genomic RAPD assay, the amplification of DNA bands show significantly change, which suggests the genome of Sasapygmaea underwent mutation after irradiation treatment. Specifically, the minimum extent of genetic mutation is 5 Gy radiation dosage group and the maximum is 25 Gy radiation dosage group. Our finding indicates that ^60Co-γ-radiation can induce dwarf plant accompanying with genomic mutation. This study lays a foundation for further breeding of Sasapygmaea via radiation.

关 键 词：^60CO-Γ射线辐照 翠竹 RAPD 矮化 白化 

分 类 号：S795[农业科学—林木遗传育种]