血浆游离DNA含量及其完整性检测对肝癌的诊断价值  

Plasma cell-free DNA level and integrity as biomarkers to diagnose hepatocellular carcinoma

作　　者：孙朝晖[1] 李林海[1] 杨永泉[1] 唐荣芝[1] 冯玉莉 

机构地区：[1]广州军区广州总医院检验科,广州510010 [2]深圳宝安区中医院检验科,深圳518000

出　　处：《解放军医学杂志》2016年第12期1016-1019,共4页Medical Journal of Chinese People's Liberation Army

基　　金：广东省医学科研基金(A2007477);广东省自然科学基金面上项目(7000065)~~

摘　　要：目的比较实时荧光定量PCR(RT-PCR)对肝细胞癌(HCC)、乙型病毒性肝炎(简称乙肝)患者及健康体检者血浆游离DNA含量及完整性的检测结果,探讨实时荧光定量PCR(RT-PCR)对HCC的诊断价值。方法收集52例HCC患者、58例乙肝患者和60例健康体检者的静脉血标本,采用RT-PCR技术对各组血浆游离DNA中GAPDH、β-actin400、β-actin167含量及其完整性(β-actin400/β-actin167)进行定量检测,结果采用SPSS软件进行统计学分析。结果健康对照组的血浆游离DNAβ-actin167含量和完整性相比明显低于HCC组(Z=–4.328,P=0.000;Z=–3.885,P=0.001)和乙型肝炎病毒(HBV)组(Z=–2.473,P=0.005;Z=–3.295,P=0.013),且HBV组血浆游离DNA完整性明显低于HCC组(Z=–4.836,P=0.002)。结论 RT-PCR检测血浆游离DNA完整性(β-actin400/β-actin167)可作为HCC辅助诊断的分子生物学指标。Objective To detect the circulating cell-free DNA （cf-DNA） level and integrity in plasma of the patients with hepatocellular carcinoma （HCC）, HBV and healthy controls by real time PCR （RT-PCR）, evaluate their diagnostic value in hepatocellular carcinoma. Methods Venous blood samples from 52 HCC patients, 58 HBV patients and 60 healthy controls were collected. The levels of glyceraldehyde-3-phosphate dehydrogenase （GAPDH）,β-actin400 and β-actin167 in plasma cf-DNA and the integrity of cf-DNA （reflected by β-actin400/β-actin167） were determined by RT-PCR. The differences in different groups and between two groups were analyzed with SPSS software. Results The β-actin167 levels in plasma cf-DNA and the integrity of cf- DNA were significantly lower in healthy controls than in HCC patients （Z=-4.328, P=0.000; Z=-3.885, P=0.001） and HBV patients （Z=-2.473, P=0.005; Z=-3.295, P=0.013）, and the integrity of cf-DNA was obviously lower in HBV patients than in HCC patients （Z=-4.836, P=0.002）. Conclusion The integrity of circulating cf-DNA β-actin167 and β-actin400 detected by RT-PCR may be auxiliary biomarker in diagnosis of hepatocellular carcinoma.

关 键 词：血浆游离DNA 癌  肝细胞 乙型肝炎 

分 类 号：R735.7[医药卫生—肿瘤]