熊果酸对成牙骨质细胞增殖及矿化功能的影响  

Effects of Ursolic Acid on Proliferation and Mineralization of Cementoblasts

作　　者：王玉琢[1,2] 包幸福[1,2] 姜欢[1,2] 高尚[1,2] 刘金凤[3,2] 王春晖[1,2] 胡敏[1,2] 

机构地区：[1]吉林大学口腔医学院正畸科,吉林长春130021 [2]吉林省牙发育及颌骨重塑与再生重点实验室,吉林长春130021 [3]吉林大学口腔医学院牙体牙髓病科,吉林长春130021

出　　处：《口腔医学研究》2016年第12期1229-1233,共5页Journal of Oral Science Research

基　　金：国家自然科学基金资助项目(编号:81470764);吉林省产业技术研究与开发项目(编号:2013C025-2)

摘　　要：目的:观察熊果酸对鼠成牙骨质细胞系OCCM-30增殖、周期、凋亡及矿化功能的影响。方法:一定浓度熊果酸处理成牙骨质细胞后,cck-8法检测细胞增殖,流式细胞仪检测细胞周期及凋亡,微量酶标法检测碱性磷酸酶活性,茜素红染色法定量及定性检测矿化结节量。结果:熊果酸组(2.5μmol/L)相比于对照组,细胞增殖率和细胞周期增殖指数升高,早期凋亡率降低,碱性磷酸酶活性和矿化结节量上调。结论:一定浓度熊果酸可促进成牙骨质细胞增殖,G1期百分比降低,抑制早期凋亡,增强碱性磷酸酶活性,促进矿化结节的形成。Objective：To investigate the effect of ursolic acid on murine cementoblasts cell line OCCM-30 in vitro.Methods：Cementoblasts were incubated with ursolic acid at various concentrations.Proliferation was detected using cck-8assay.Cell cycle and apoptosis were checked by flow cytometry analysis.The activity of alkaline phosphatase was tested by enzyme-linked method on microplate.The mineralization was tested by alizarin red S staining.Results：Compared with the control group,ursolic acid（2.5μmol/L）increased the cell proliferation rate and proliferation index of the cell cycle,reduced the early apoptotic rate,up-regulated the activity of alkaline phosphatase and the quantity of the mineralized nodules.Conclusion：Ursolic acid can promote the proliferation of cementoblast,enhance the activity of alkaline phosphatase,and facilitate the differentiation.

关 键 词：熊果酸 鼠成牙骨质细胞 OCCM-30 牙根吸收与修复 

分 类 号：R783.5[医药卫生—口腔医学]