基质用量和料水比对蛹虫草子座虫草素产量的影响  

Effect of Substrate Dosage and Substrate-to-Water Ratio on Yield of Cordycepin in Cordyceps militaris

作　　者：简利茹[1] 杜双田[2] 蒋琪[3] 张园园[4] 

机构地区：[1]西北农林科技大学,旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [2]西北农林科技大学生命科学学院,陕西杨凌712100 [3]吉林大学食品科学与工程学院,长春130062 [4]陕西省安康市农业科学研究所,陕西安康725000

出　　处：《西北农业学报》2016年第11期1736-1741,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省科技统筹创新工程计划(2011KTCL02-16)~~

摘　　要：探索基质用量和料水比对蛹虫草子座中虫草素产量的影响,为蛹虫草的优质栽培提供依据。以蛹虫草菌株CM-16为研究对象,小麦为主要栽培基质,采用单因素和二次正交旋转组合设计2种分析法,研究基质用量和料水比对蛹虫草子座中虫草素产量的影响。结果表明,随着基质用量和料水比的增加,蛹虫草子座中虫草素的产量均呈先增加后降低的趋势,当基质用量为每盒350g,料水比(质量体积比)控制在1∶1.5时,蛹虫草子座中虫草素质量分数最高,为3.96mg/g。基质用量和料水比是影响蛹虫草子座中虫草素质量分数的重要因素,当以小麦为主要原料栽培蛹虫草时,小麦用量为每盒350g,最佳料水比(质量体积比)控制在1∶1.5。This study aims to investigate the effects of substrate dosage and substrate-to-water ratio in the medium on production of cordycepin in the stroma of Cordyceps militaris,and further provide scientific basis for C.militaris cultivation.Cordyceps militaris strain CM-16 and wheat were used as material and main cultivation medium,respectively.Single factor and two orthogonal rotation combination methods were used to test the effects of substrate dosage and medium substrate-to-water ration on cordycepin content in the stroma.The results showed that the increasing of substrate dosage and medium substrate-to-water ratio,the cordycepin content in stroma increased initially,then decreased.The cordycepin content in stroma reached its maximum：3.96 mg/g when the substrate dosage was350 g per box and medium substrate-to-water ration was 1∶1.5.In conclusion,the ubstrate dosage and medium substrate-to-water play an important role of the yield of cordycepin in stroma of C.militaris.When wheat was used as the main cultivation medium,the optimum substrate dosage and medium substrate-to-water ration were 350 g per box and 1∶1.5,respectively.

关 键 词：蛹虫草 栽培基质 料水比 虫草素 

分 类 号：S567.35[农业科学—中草药栽培]