可降解三乙胺的赤红球菌S6-2的筛选与鉴定及降解特性  

Screening,Identification and Characterization of a Triethylamine Degrading Bacterium Rhodococcus ruber S6-2

作　　者：孙磊[1,2] 宋彤彤[1] 朱珍妮[1] 李丹阳[1] 刘存歧[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]河北省微生物多样性研究与应用实验室,河北保定071002

出　　处：《环境科学研究》2016年第12期1882-1886,共5页Research of Environmental Sciences

基　　金：国家水体污染控制与治理科技重大专项(2012ZX07203003);河北省生物工程重点学科项目

摘　　要：为治理制药废水中残留的难降解有机污染物TEA(triethylamine,三乙胺),以石家庄某污水处理厂的活性污泥为材料,采用富集培养和选择培养,分离筛选到1株能以TEA为唯一碳源和氮源生长代谢的降解菌——S6-2.通过测定形态特征、生理生化特性、G+C(碱基对)摩尔百分比及16S rRNA基因序列系统发育分析,该菌株被鉴定为赤红球菌(Rhodococcus ruber).采用单一影响因素试验分析菌株S6-2对TEA的降解特性,结果表明:菌株S6-2具有较强的TEA降解能力及降解稳定性;其最适降解温度为32℃,最适降解pH为8.0.菌株S6-2可耐受较高浓度TEA,在ρ(TEA)为900 mg/L的培养基中仍能生长;在最适条件下,菌株S6-2对TEA的降解率为70.7%±1.8%.该株菌在含TEA的无机盐平板上传代培养15代后,对TEA的降解率为69.3%±2.5%,说明菌株S6-2对TEA的降解具有稳定性.研究显示,菌株S6-2可作为TEA污染水体生物修复的潜在资源.In order to treat wastewater contaminated by triethylamine,which is generated from pharmaceutical factories,bacterial strains which could degrade triethylamine were screened. A bacterial strain S6-2 that used triethylamine as the sole carbon and energy source was isolated from the activated sludge of a wastewater treatment plant in Shijiazhuang by enrichment and selective medium. S6-2 was identified as Rhodococcus ruber by morphological,physiological and biochemical characteristics,as well as（ G ＋ C） mol percentage and 16 S rRNA gene phylogenic analysis. Through single-factor experiments of degrading features,S6-2 was shown to have high degradation ability and stability. The study demonstrated that temperature and pH were important factors for triethylamine degradation. The optimal temperature was 32 ℃, and the optimal pH was 8. 0. This strain was tolerant to a high concentration oftriethylamine and grew normally in tradethylamine as high as 900 mg/L. Under the optimal conditions,the triethylamine degradation rate reached 70. 7% ± 1. 8%. After 15 generations subcultured on mineral salt plates supplemented with triethylamine,the triethylamine degradation rate was 69. 3% ± 2. 5%.The results showed that S6-2 had steady ability of triethylamine degradation,and suggested that S6-2 could be a potential strain for treating triethylamine-contaminated wastewater.

关 键 词：三乙胺 生物降解 赤红球菌 分离鉴定 

分 类 号：X172[环境科学与工程—环境科学]