两个玉米自交系对生平脐蠕孢菌的抗病相关反应比较  

Comparison of Defense-Related Responses Induced by Bipolariszeicolain Two Maize Inbred Lines

作　　者：常小丽[1,2] 戴浩[2] 张小玲[2] 陈磊[2] 孙小芳[2] 祁小波[2] 

机构地区：[1]四川农业大学生态农业研究所,成都611130 [2]四川农业大学农学院植物病理系,成都611130

出　　处：《西南大学学报（自然科学版）》2016年第12期1-9,共9页Journal of Southwest University(Natural Science Edition)

基　　金：四川省教育厅项目(13ZB0278);四川省科技支撑项目(2015NZ0040)

摘　　要：试验以西南地区两个优良玉米自交系R08与18-599R为材料,采用苗期人工接种玉米生平脐蠕孢菌（Bipolariszeicola）进行抗性鉴定,并对接种后自交系叶片中的活性氧（ROS）、抗氧化物酶类（SOD,POD,CAT）活性、丙二醛（MDA）质量分数、抗病相关基因（PR1,ZmDREB2A和ZmERF3）表达及过敏性反应（HR）等抗病相关反应进行了检测,为玉米抗病育种提供依据.结果表明：① R08对B.zeicola表现感病,18-599R为高感;②叶片受侵染后,ROS在R08中迅速且大量地积累,而在18-599R相对滞后且量少;③ POD,SOD,CAT活性随接种时间呈上升趋势,在接种后12~24h左右出现峰值,随后降低,但其增幅存在差异,同时,MDA质量分数在R08中比18-599R更高;④3个基因于接种早期在18-599R中微量表达后均被抑制,而R08在12~24h大量表达,随后降低;⑤ R08叶片在接种后出现严重的HR反应,而18-599R相对较轻.In this study, two elite inbred maize cultivars R08 and 18-599R was inoculated with the cultured B. zeicola, and the seedling resistance was evaluated, and meanwhile, several defense-related responses including reactive oxygen species （ROS）, antioxidant enzymes （Peroxidase, PODl Superoxide dismutase, SODl Catalase, CAT）, contents of malonaldehyde （MAD）, defense gene expression （PR1, ZmDREB2A and ZrnERF3） and hypersensitive reaction （HR） were examined on the detached leaves after inoculation with B. zeicola over time. We found that R08 showed susceptible to B. zeicola, whereas 18-599R was high susceptible. ROS was induced in a much higher on R08 than those on 18-599R. A rapid increase of ac- tivities of POD, SOD, and CAT were examined, respectively, peaked at around 12~24 h, and then grad- ually decreased, however, there was a significant difference in the increased attitude of three antioxidant enzymes. The content of MDA was commonly much higher on 18-599R than that on R08. In addition, two maize inbred lines showed significant difference in defense gene expression of PR1, ZmDREB2A and ZrnERF3, which were a little expressed and then suppressed on 18-599R, but largely induced at 12~24 h. Furthermore, a high and strong HR on R08 was induced as compared to 18-599R. This study demonstrates that R08 and I8-599R showed different resistance to B. zeicola on different defense-related responses, and this provides a basis for maize resistance breeding as well as for effective control of Northern Corn Leaf Spot disease.

关 键 词：玉米自交系 Bipolariszeicola 抗氧化物酶 抗病基因 过敏性反应 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]