白藜芦醇诱导Hep-2人喉表皮样癌细胞自噬作用  

Effect of Resveratrol on Autophagy of Hep-2 Cell

作　　者：王敏[1] 赵红[2] 郑家法[1] 谢远杰[1] 

机构地区：[1]南华大学附属南华医院耳鼻喉科,湖南省衡阳市421002 [2]南华大学护理学院基础护理教研室

出　　处：《医学理论与实践》2016年第22期3021-3023,共3页The Journal of Medical Theory and Practice

基　　金：湖南省医药卫生基金资助项目(B2013-037)

摘　　要：目的:观察白藜芦醇对Hep-2细胞形态学的影响及诱导其自噬的可能机制。方法:40μM浓度白藜芦醇复合培养液作用于Hep-2细胞后,MDC染色和透射电镜分别检测自噬小体和自噬泡;Hoechest33258染色检测凋亡细胞;western blot分别分析自噬基因蛋白水平。结果:白藜芦醇增加LC3Ⅱ与LC3Ⅰ的比率,促进beclin1蛋白和mRNA的表达,细胞内的自噬泡和自噬小体数量也显著增加。用3MA和CQ抑制自噬则白藜芦醇对Hep-2细胞的促凋亡作用显著减弱。结论:白藜芦醇能够促进Hep-2细胞自噬;白藜芦醇诱导Hep-2细胞自噬的机制可能与促进LC3Ⅱ与LC3Ⅰ的表达、增强自噬基因beclin1蛋白的表达有关。Objective：To explore the effects of resveratrol promoting Hep-2cells death by induction autophagy.Methods：We apply Western blotting to detect the protein.The autophagic bodies and autophagic vacuoles were detected by MDC staining and transmission electron microscope respectively.Apoptotic cell within the Hep-2cells was examined by Hoechest33258 staining.All data are analyzed by using SPSS13 software.Results：Resveratrol can increase the ratio of LC3Ⅱand LC3Ⅰ,promoting the protein expression of beclin-1and the number of autophagic vacuoles and autophagosomes were also increased significantly.The promotion role of resveratrol on apoptosis in Hep-2cells was weakened significantly with using 3MA and CQ inhibiting autophagy.Conclusion：Resveratrol can promote Hep-2cells autophagy.The promoting function of resveratrol on Hep-2cells apoptosis is decreased when inhibit autophagy.

关 键 词：白藜芦醇 人喉表皮样癌细胞 自噬 

分 类 号：R739.65[医药卫生—肿瘤]