自动固相萃取-气相色谱串联质谱法测定果蔬中的多组分农药残留  

Determination of multi-component pesticide residues in fruits and vegetables by automatic solid phase extraction and gas chromatography tandem mass spectrometry

作　　者：徐燕[1] 王志强[1] 乔永青[1] 王余萍[1] 王冬梅[1] 

机构地区：[1]安阳市疾病预防控制中心,河南安阳455000

出　　处：《实用预防医学》2017年第1期117-119,共3页Practical Preventive Medicine

摘　　要：目的建立一种同时测定果蔬中有机磷、氨基甲酸酯类及拟除虫菊酯类等多组分农药残留的分析方法。方法果蔬样品经乙腈提取,无水硫酸钠脱水,固相萃取小柱净化,再经旋转蒸发及氮吹浓缩后,用GC-MS/MS进行测定,外标法定量。结果有机磷、氨基甲酸酯类及拟除虫菊酯类等多组分农药经过36 min后完全分离,在0.01~10.0μg/ml的线性范围内相关系数r均>0.9956,平均加标回收率在72.1%~109.3%之间,相对标准偏差在4.0%~11.3%之间。结论该方法不仅前处理简便易操作,而且具有较高的灵敏度、准确度和精密度,适合于大批量样品的日常检测。Objective To establish an analytical method for simultaneous determination of multi-component pesticide residues in fruits and vegetables by automatic solid phase extraction and gas chromatography tandem mass spectrometry. Methods Fruit and vegetable samples were extracted with acetonitrile, dehydrated by anhydrous sodium sulfate, purified by solid phase extraction small column, performed rotary evaporation and nitrogen blow after enrichment, determined by GC-MS/MS, and quantitated by external standard method. Results Multi-component pesticides were separated completely after 36 min. The correlation coefficient r was 〉0. 9956 within the linear range of 0.01-10.0 μg/ml. The average standard addition recovery rates were between 72.1% amd 109. 3%, and the relative standard deviation was between 4.0% and 11.3%. Conclusions This method not only has the advantage of simple pretreatment, but also shows higher sensitivity, accuracy and precision. It is suitable for routine detection of bulk samples.

关 键 词：固相萃取 气相色谱串联质谱 果蔬 多组分农药残留 

分 类 号：R155.5[医药卫生—营养与食品卫生学]