RP-HPLC测定红曲菌丝体中辅酶Q_(10)的研究  

Coenzyme Q_(10) Determination in Monascus mycelium by RP-HPLC

作　　者：吕思敏 孙金影 于琼 丁喜生 崔燎[3] 吴铁 

机构地区：[1]广东医科大学.广东润和生物科技有限公司辅酶Q10联合研究中心,广东东莞523808 [2]广东医科大学药学院,广东东莞523808 [3]广东天然药物研究与开发重点实验室,广东湛江524023

出　　处：《广东化工》2016年第23期103-105,共3页Guangdong Chemical Industry

基　　金：广东医科大学.广东润和生物科技有限公司共建辅酶Q10联合研究中心项目(2XX14002)

摘　　要：用反相高效液相色谱测定红曲菌丝体中辅酶Q10的含量。采用超声破碎细胞方法提取红曲菌丝体中辅酶Q10,色谱柱为Eclipse Pius C18（4.6＊250 mm,5μm）,流动相为甲醇∶无水乙醇=（V∶V）50∶50;流量为1 mL/min;进样体积为10μL;检测波长为275 nm;柱温为30℃。辅酶Q10在4.4453125～71.125？g/mL浓度范围内线性关系良好,回归方程为Y=8.7180X-4.7213,R^2=0.9999,平均加标回收率为66.29%,RSD为2.41%。该法具有良好的线性关系,重现性好、精密度高、准确度好,可用于红曲菌丝体中辅酶Q10的含量测定。A reversed-phase HPLC method was established for coenzyme Q10 determination in monascus mycelium. The coenzyme Q10 in mycelia of monascus was extracted by ultrasonic disruption, and coenzyme Q10 was detected using Eclipse Pius C18（4.6 ＊ 250 mm, 5 mm） and methanol∶ethanol=50∶50 as mobile phase at a flow rate of 1 mL/min and column temperature was kept at 30 ℃. And sample of 10 μL was injected into the instrument for analysis at a wavelength of 275 nm. Coenzyme Q10 was lined in the concentration range of 4.4453125～71.125 μg/mL with the linear equation of Y=8.7180X-4.7213（R^2=0.9999）. The average recovery of coenzyme Q10 was 66.29 %（RSD=2.41 %）. The method for coenzyme Q10 determination of Monascus mycelium has higher linearity, reproductive, precision, and accurate.

关 键 词：RP-HPLC 红曲菌丝体 辅酶Q10 

分 类 号：TQ925.9[轻工技术与工程—发酵工程]