Preparation of Functionalized Fe304@SiO2 MagneticNanoparticles for Monoclonal Antibody Purification  

作　　者：HOU Xuemei ZHAO Changjie TIAN Yanlong DOU Shuliang ZHANG Xiang ZHAO Jiupeng 

机构地区：[1]School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin 150001, P. R. China [2]Center for Composite Materials, Harbin Institute of Technology, Harbin 150001, P. R. China

出　　处：《Chemical Research in Chinese Universities》2016年第6期889-894,共6页高等学校化学研究（英文版）

摘　　要：Magnetic Fe304@SiO2 nanoparticles with superparamagnetic properties were prepared via a reverse mi-croemulsion method at room temperature. The as-prepared samples were characterized by transmission electron mi-croscopy（TEM）, X-ray diffractometry（XRD）, and vibrating sample magnetometry（VSM）. The Fe304@SiO2 nanopar-ticles were modified by （3-aminopropyl）triethoxysilane（APTES） and subsequently activated by glutaraldehyde（Glu）.Protein A was successfully immobilized covalently onto the Glu activated Fe304@SiO2 nanoparticles. The adsorptioncapacity of the nanoparticles was determined on an ultraviolet spectrophotometer（UV） and approximately up to 203mg/g of protein A could be uniformly immobilized onto the modified Fe304@SiO2 magnetic beads. The core-shell ofthe Fe304@SiO2 magnetic beads decorated with protein A showed a good binding capacity for the chime-ric anti-EGFR monoclonal antibody（anti-EGFR mAb）. The purity of the anti-EGFR mAb was analyzed by virtue ofHPLC. The protein A immobilized affinity beads provided a purity of about 95.4%.

关 键 词：Magnetic Fe304@SiO2 Protein A Nanoparticle MONOCLONAL ANTIBODY 

分 类 号：O[理学]