Determination of Cysteine Using Near-infrared Diffuse Reflectance Spectroscopy with Enrichment via Thiol-maleimide Click Reaction  

作　　者：WANG Cuicui CAI Wensheng SHAO Xueguang 

机构地区：[1]Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, P. R. China [2]Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin 3000 71, P. R. China [3]State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, P. R. China [4]Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, Tianjin 300071, R R. China

出　　处：《Chemical Research in Chinese Universities》2016年第6期912-916,共5页高等学校化学研究（英文版）

摘　　要：Enrichment technique has been proved to be an efficient way to make the near-infrared diffuse reflectancespectroscopy（NIRDRS） suitable for micro analysis. However, low selectivity presented by conventional enrichmentmethods makes the quantitative analysis easy to be affected by the coexisting components. In this study, a specificenrichment method with chemical bonding via thiol-maleimide click reaction was used to achieve the reduction of theinterferences. Taking cysteine as the analyzing target, maleimide-functionalized SiO2 nanoparticles were prepared forthe enrichment of cysteine. Then determination of cysteine in aqueous solution and human serum was studied usingthe partial least squares model built from the NIRDRS spectra of the adsorbate. The results show that the concentra-tion that can be quantitatively detected is as low as 2.0 μg/mL, and the correlation coefficient（R） between thereference and predicted concentration is 0.9871 for the validation samples. The recoveries are in the range of89.5%-113.8% for human serum samples in the concentration range of 0--16.2 μg/mL.

关 键 词：Near-infrared DIFFUSE reflectance spectroscopy CLICK reaction CYSTEINE Multivariate calibration Humanserum 

分 类 号：O[理学]