苎麻根际土壤浸提物的气相色谱-质谱(GC-MS)检测分析  

GC-MS Analysis of Rhizosphere-Soil Extract of Ramie

作　　者：白玉超[1,2] 杨瑞芳[1] 刘楠楠[1] 李雪玲[1] 李林林[1] 佘玮[1] 崔国贤[1,2] 

机构地区：[1]湖南农业大学苎麻研究所,长沙410128 [2]中国农业科学院麻类研究所,长沙410205

出　　处：《中国麻业科学》2016年第6期296-302,共7页Plant Fiber Sciences in China

基　　金：国家麻类产业技术体系(CARS-19-E20);国家自然科学基金(31471543);中国农业科学院科技创新工程(ASTIP-IBFC07);湖南省研究生科研创新项目(CX2015B232)

摘　　要：对苎麻根际土壤浸提液进行了检测分析,以对苎麻根系分泌物的组成进行初步探索。以乙酸乙酯和正己烷为浸提剂浸提"湘苎3号"、"多倍体1号"、"中苎1号"、"湘苎7号"和"R057"根际土壤,浸提液通过过滤、浓缩,用气相色谱—质谱联用仪(GC-MS)进行了组分分析。"湘苎3号"、"多倍体1号"、"中苎1号"、"湘苎7号"和"R057"根际土壤乙酸乙酯浸提液中物质相对含量分别为4.77%、3.94%、3.48%、3.86%和4.19%,正己烷浸提液中物质相对含量分别为6.93%、5.58%、5.54%、7.11%和6.29%。苎麻根际土壤乙酸乙酯和正己烷浸提液中分别检测并鉴定了20、12种物质,主要包括烷烃类、酯类、酸类等,含量相对较高的有棕榈酸、油酸酰胺、2-甲基二十烷、正二十七烷等。其中棕榈酸是典型的化感物质,其对苎麻生长产生的影响及作用机制有待进一步研究。Rhizosphere soil extract was analyzed to acquire primary information about dates in this paper. Ethyl acetate and n -hexane were used to extract rhizoshpere soil of ramie root exu- Xiangzhu - 3, Tri - 1, Zhongzhu - 1, Xiangzhu - 7 and R057. The extract was filtrated and concentrated, then ana- lyzed by GC - MS. The relative content of the organic ingredients in ethyl acetate extracts of Xiangzhu - 3, Tri - 1, Zhongzhu - 1, Xiangzhu -7 and R057 was 4. 77% , 3.94% , 3.48%, 3.86% and 4. 19% , and in n -hexane extracts was 6. 93% , 5.58% , 5.54%, 7.11% and 6. 29% , respectively. About 20 peaks were observed and identified from ethyl acetate and 12 from n - hexane extracts, which consisted of hydrocarbon, ester, organic acid, and so on. The comparatively higher proportions with relative components were palmitie acid, oleamide, 2 - methyleicosane, heptacosane, and so on. The palmitie acid was a kind of allelochemical, and its impact on ramie growth and action mechanism needs to be further studied.

关 键 词：苎麻 根际土 气相色谱-质谱 

分 类 号：S563.1[农业科学—作物学]