群体感应淬灭菌的分离及其膜污染控制性能  

Isolation of Quorum Quenching Bacteria and Their Function for Controlling Membrane Biofouling

作　　者：赵畅[1] 王文昭[1] 徐期勇[1] 

机构地区：[1]北京大学深圳研究生院环境与能源学院,聚硅酸盐复合环保材料工程实验室,深圳518055

出　　处：《环境科学》2016年第12期4720-4726,共7页Environmental Science

基　　金：深圳市科技计划项目(JCYJ20150626110817181,JCYJ20150806112326712,CXZZ20151117141320317)

摘　　要：通过淬灭细菌的群体感应系统来抑制生物膜形成、防止膜生物污染的方法近年来受到广泛关注.本实验从实际运行污水处理厂活性污泥中分离出5株具有群体感应淬灭功能的菌株,其中菌株HG10对信号分子N-乙酰高丝氨酸环内酯(C6-HSL)分解能力最强.经16S rRNA基因序列比对,初步鉴定为蜡样芽孢杆菌(Bacillus cereus).用海藻酸钠将菌株HG10进行包埋固定,以探究其在膜过滤系统中对膜污染防治的效果.结果表明,经过8 d培养,添加细菌包埋珠(SA-HG10)的实验组B中膜通量为181.29 L·(m^2·h)^(-1),未投加包埋珠的对照组A膜通量为110.64 L·(m^2·h)^(-1),B组膜通量比A组高出63.86%;对微滤膜片上生物膜中EPS含量测定表明,实验组B中EPS多糖和蛋白质含量较对照组A分别减少了29%和48%,疏水性蛋白质含量的大量减少是造成膜污染减弱的主要原因;膜表面胞外聚合物(EPS)总含量减少了43%,表明投放SA-HG10细菌包埋珠对过滤膜片上生物膜形成具有明显抑制作用,改善了膜过滤性能.Interspecies quorum quenching by bacterial cells has been reported as a novel approach for mitigating the biofouling via restraining quorum sensing（ QS）. Five indigenous quorum quenching bacterial strains were isolated from activated sludge taken from wastewater treatment plant. Strain HG10 showed high degrading activity against C6-HSL（ N-hexanoyl-L-Homoserine lactone）. The result of 16 S r DNA sequencing showed that the isolated strain HG10 belonged to the genus Bacillus cereus. Strain HG10 was immobilized in sodium alginate（ SA）, and the biofouling inhibition of SA-HG10 in membrane filtration treatment system was investigated. The results showed that the membrane flux in experimental group B（ provided with SA-HG10） was 181. 29 L·（ m^2·h）^-1,and the membrane flux in control group A（ without the beads） was 110. 64 L·（ m^2·h）^-1,The membrane flux in group B was 63. 86%higher than group A. The content of extracellular polymeric substances（ EPS） in the biofilm on the membrane filter was also measured. The results showed that the contents of polysaccharides and proteins in the experimental group B decreased by 29% and48% respectively than those of group A. A large decrease in the content of hydrophobic proteins was the main reason for the decrease of membrane fouling. The content of EPS in the membrane surface decreased by 43%,indicating that SA-HG10 could inhibit biofilm formation on the membrane filter and effectively improve the filtering performance.

关 键 词：群体感应淬灭 蜡样芽孢杆菌 固定化 膜污染 胞外聚合物 

分 类 号：X131.2[环境科学与工程—环境科学] X172