北京市不同地区土壤中的球囊霉素荧光特征及其与土壤理化性质的关系  

Fluorescence Properties of Glomalin and Its Relationship with Soil Physyicochemical Characteristics in Different Regions of Beijing City

作　　者：柴立伟 刘梦娇[2] 蒋大林[1] 樊灏 曹晓峰[2] 黄艺[1] 

机构地区：[1]北京大学环境科学与工程学院,环境模拟与污染控制国家重点实验室,北京100871 [2]北京大学深圳研究生院,深圳518055

出　　处：《环境科学》2016年第12期4806-4814,共9页Environmental Science

基　　金：国家自然科学基金项目(41271325)

摘　　要：球囊霉素相关土壤蛋白是一种菌根真菌产生的耐热糖蛋白,因其与微生物密切联系而常用来表征土壤的健康状况.由于球囊霉素相关土壤蛋白的提取过程中容易产生干扰物质,影响了这个指标在土壤状态评估方面的进一步应用.本研究采集了北京北部山区,中部城区以及南部郊区的土壤样品,使用三维荧光-平行因子模型技术解析球囊霉素相关土壤蛋白中可溶性有机物的荧光特征,将土壤类球囊霉素蛋白分解为5个荧光组分,分别为微生物源UVC腐殖质类物质、UVA人为来源腐殖质类物质、微生物来源的氧化醌物质、土壤富里酸类物质和类蛋白物质,蛋白质在其中所占比例为0-20%.不同采样区域的荧光特征比较显示,球囊霉素土壤相关土壤蛋白的5个荧光组分总体稳定,与山区相比较,城区和郊区的类蛋白质组分受人类活动影响降低,氧化醌类物质组分含量升高.不同采样点的理化特征结果显示,以城市建设和耕作为代表的人类活动减少了土壤中的含水率、有机质含量以及总氮含量.比较球囊霉素相关土壤蛋白的荧光特征与土壤理化特征的相关性结果显示,球囊霉素土壤相关蛋白浓度、UVA腐殖质组分、UVC腐殖质组分与土壤有机质含量、总氮含量显著相关,具有表征土壤健康状况的潜力.Glomalin-related soil proteins（ GRSP）,which has been used as a presumable biological indicator of soil quality,is of vital ecological importance. In this research,we tested the GRSP content and physicochemical property of soils collected in mountain,urban and suburb areas of Beijing City. Besides,the fluorescence property of GRSP was also studied by using EEM-PARAFAC. The results showed that GRSP could be decomposed into five compounds,terrestrial humic-like substances UVA,terrestrial humic-like substances UVC,oxidized quinones,soil fulvic acid and amino acids. The GRSP content and protein-like fluorescence substances in urban and suburb areas significant decreased and oxidized quinones content elevated in comparison with those in mountain area. The physicochemical property was also shaped by urbanization. Compare to mountain area,moisture content,total organic matters and total nitrogen significantly decreased in urban area soil and suburb area. GRSP content,terrestrial humic-like substances UVA and oxidized quinones content showed significant correlation with soil organic matter and total nitrogen. Base on that,an integrated index system mainly consisting of GRSP concentration and C1 component and C3 component was recommended to evaluate the soil health status.

关 键 词：城市土壤 三维荧光 平行因子分析 土壤理化性质 球囊霉素相关土壤蛋白 

分 类 号：X171.1[环境科学与工程—环境科学]