ABTS分光光度法在饮用水消毒剂检测中的应用进展  

Research review over the application advances of the spectrophotometric determination of the commonly used in drinking water disinfectants with ABTS

作　　者：王涛[1] 彭道平[1] 李云祯[2] 白杨[1] 贺玉龙[1] 黄涛[1] WANG Tan PENG Dan-ping LI Yun-zhen BAI Yang HE Yu-long HUANG Tan(Faculty of Geosciences and Environmental Engineering, Southwest Jiantong University, Chengdu 611756, China Sichuan Academy of Environmental Science, Chengdu 610041, China)

机构地区：[1]西南交通大学地球科学与环境工程学院,成都611756 [2]四川省环境保护科学研究院,成都610041

出　　处：《安全与环境学报》2016年第6期326-331,共6页Journal of Safety and Environment

基　　金：国家建设高水平大学公派研究生项目(201207000022);教育部新世纪优秀人才支持计划项目(NCET-11-0710)

摘　　要：2,2-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐（ABTS）可与水体中的消毒剂反应生成绿色稳定自由基ABTS.＋,其在405815 nm范围内有5处可用吸收峰（405 nm、415 nm、649 nm、732 nm、815 nm）,因此,ABTS分光光度法在近几年被广泛应用于检测水体中的消毒剂。详细介绍了ABTS分光光度法对水体中多种消毒剂（HOCl、NH2Cl、ClO2、HOBr、NH2Br、NHBr2、NBr3、KMnO4、Fe（Ⅵ）、PAA、DBDMH、O3）的检测原理和操作步骤,并与传统检测方法（直接分光光度法、DPD分光光度法、碘量法等）进行了对比,归纳了ABTS分光光度法的优势,对ABTS替代原有检测方法的可能性及应用前景进行了展望。结果表明,ABTS分光光度法具有操作简单、步骤少（通常只需1步）、使用常规仪器（紫外/可见光分光光度计）、不使用重金属及有毒试剂、检测限低（通常小于2μg/L）、抗干扰能力强（有多处吸收峰,可避开干扰）、产物ABTS.＋稳定性好的特点,可完全或部分取代原有水体消毒剂检测方法。ABTS分光光度法可以应用于实验室、水厂、污水处理厂及其他领域中的水体消毒剂检测。The present paper intends to introduce new methods for identifying and determining the disinfectants commonly existing in the water with ABTS （ 2,2＇-azino-bis （ 3-ethylbenzothiazoline-6-sulfonate）. The above mentioned methods for drinking water disinfectants determination and identification have been recommended by the American Public Health Association （APHA）and the Chinese Standard Examination Methods （CSEM）, such as UV/Vis spectrophotometric methods, the N, N-diethyl-l,4-phe- nylenediamine（DPD） spectrophotometric methods, the iodometric methods, the indigo spectrophotometric methods, and chromato- graphic methods, have been developed and used for quite a long time, though there may still remain some drawbacks in the actual application. For example, since ABTS is a colorless anti-oxidant substrate for enzyme peroxidase, which can be oxidized to a kind of green color product ABTS ＋, the said ABTS＋ can thus be detected by testing their variety of wavelengths. Practically speaking, it is possible for us to observe and detect at least five broad peaks in its spectrum. The highest absorbance of the said ABTS can be found at 415 nm, however, the other four wavelengths can be made available for their measurements. In accordance with the aforementioned characteristics of ABTS, we have first briefly illustrated the principles and the operation procedures of the recommended methods. And, then, it would be possible for us to describe in detail the principles and operating steps of the said ABTS spectrophotometric methods for determining the chlorine species, the chlorine dioxide, bromine species, bromate, ferrate （ VI ）, potassium permanganate, ozone, and the peroxycarboxylic acid（ PAA） in the aquatic condition. And, next, we begin to illustrate the precision, the detection limits, the mo- lar absorptivities, the operation steps, and the antijamming capa- bilities among the recommended methods in addition to the ABTS spectrophotometric methods through comparison of the paramet

关 键 词：环境学 ABTS 分光光度法 消毒剂 饮用水 

分 类 号：X1[环境科学与工程—环境科学]