Distinct roles of ASIC3 and TRPV1 receptors in electroacupuncture-induced segmental and systemic analgesia  

作　　者：Juanjuan Xin Yangshuai Su Zhaokun Yang Wei He Hong Shi Xiaoyu Wang Ling Hu Xiaochun Yu Xianghong Jing Bing Zhu 

机构地区：[1]Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beo'ing 100700, China

出　　处：《Frontiers of Medicine》2016年第4期465-472,共8页医学前沿（英文版）

基　　金：This work was supported by National Natural Science Foundation of China （Nos. 81330087 and 81503668） and Beijing Natural Science Foundation （No. 7132148）.

摘　　要：Previous studies have demonstrated the effects of different afferent fibers on electroacupuncture （EA）- induced analgesia. However, contributions of functional receptors expressed on afferent fibers to the EA analgesia remain unclear. This study investigates the roles of acid-sensing ion channel 3 （ASIC3） and transient receptor potential vanifioid 1 （TRPV1） receptors in EA-induced segmental and systemic analgesia. Effects of EA at acupoint ST36 with different intensities on the C-fiber reflex and mechanical and thermal pain thresholds were measured among the ASIC3-/-, TRPV1-/-, and C57BL/6 mice. Compared with C57BL/6 mice, the ipsilateral inhibition of EA with 0.8 C-fiber threshold （0.8Tc） intensity on C-fiber reflex was markedly reduced in ASIC3-/- mice, whereas the bilateral inhibition of 1.0 and 2.0Tc EA was significantly decreased in TRPV1-/- mice. The segmental increase in pain thresholds induced by 0.3 mA EA was significantly reduced in ASIC3-/- mice, whereas the systemic enhancement of 1.0 mA EA was markedly decreased in TRPV1-/- mice. Thus, segmental analgesia of EA with lower intensity is partially mediated by ASIC3 receptor on Aβ-fiber, whereas systemic analgesia induced by EA with higher intensity is more likely induced by TRPV1 receptor on Aδ- and C-fibers.Previous studies have demonstrated the effects of different afferent fibers on electroacupuncture （EA）- induced analgesia. However, contributions of functional receptors expressed on afferent fibers to the EA analgesia remain unclear. This study investigates the roles of acid-sensing ion channel 3 （ASIC3） and transient receptor potential vanifioid 1 （TRPV1） receptors in EA-induced segmental and systemic analgesia. Effects of EA at acupoint ST36 with different intensities on the C-fiber reflex and mechanical and thermal pain thresholds were measured among the ASIC3-/-, TRPV1-/-, and C57BL/6 mice. Compared with C57BL/6 mice, the ipsilateral inhibition of EA with 0.8 C-fiber threshold （0.8Tc） intensity on C-fiber reflex was markedly reduced in ASIC3-/- mice, whereas the bilateral inhibition of 1.0 and 2.0Tc EA was significantly decreased in TRPV1-/- mice. The segmental increase in pain thresholds induced by 0.3 mA EA was significantly reduced in ASIC3-/- mice, whereas the systemic enhancement of 1.0 mA EA was markedly decreased in TRPV1-/- mice. Thus, segmental analgesia of EA with lower intensity is partially mediated by ASIC3 receptor on Aβ-fiber, whereas systemic analgesia induced by EA with higher intensity is more likely induced by TRPV1 receptor on Aδ- and C-fibers.

关 键 词：ELECTROACUPUNCTURE ANALGESIA ASIC3 TRPV1 C-fiber reflex 

分 类 号：R[医药卫生]