胃癌组织中CapG蛋白的表达及其与临床病理特征及预后的关系  

Expression of CapG in gastric cancer and its relationship with clinicopathological features and prognosis

作　　者：吕旻桦 梁博[1] 徐康保 邹镇洪[1] 

机构地区：[1]南昌大学第二附属医院普外科,南昌330006 [2]南昌大学第一附属医院医学科研中心,南昌330006 [3]江西省吉安市永丰县人民医院,永丰331500

出　　处：《临床与实验病理学杂志》2016年第12期1329-1333,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(81560389)

摘　　要：目的检测巨噬细胞加帽蛋白(gelsolin-like actin-capping protein,CapG)在胃癌及癌旁组织(距离癌组织边缘>5 cm)中的表达,探讨CapG蛋白表达与胃癌临床病理参数及预后的关系。方法收集125例胃癌石蜡包埋组织样本及癌旁组织30例。患者均为未行放、化疗的初诊患者。每例组织样本均行CapG免疫组化染色,并设置对照组。通过病理医师评分来确定CapG蛋白的表达量,探讨CapG蛋白表达与胃癌临床病理参数及预后的关系。结果 CapG蛋白在细胞质和细胞核中均表达,呈棕黄色颗粒状。癌旁组织中基本无表达,胃癌组织中的表达显著高于癌旁组织;胃癌组织中CapG蛋白表达与患者性别、年龄、肿瘤大小、肿瘤位置无显著相关(P均>0.05),与浸润深度(P=0.044)、淋巴结转移(P=0.026)、远处转移(P=0.001)及AJCC分期(P=0.012)显著相关。Kaplan-Meier生存曲线显示,CapG蛋白高表达组患者的总体预后较低表达组差,且差异具有显著性(P<0.001)。Cox回归分析显示,肿瘤位置、淋巴结转移、远处转移、AJCC分期、CapG蛋白表达情况是胃癌患者预后的独立危险因素。结论 CapG蛋白在胃癌组织中高表达,可能与胃癌的发生、发展有关,可作为胃癌预后判断的指标之一,有望成为胃癌治疗的潜在新靶点。Purpose To investigate the expression of gelsolin-like actin-capping protein （CapG） in gastric cancer and adjacent normal gastric tissues and its relationship with clinicopathological features and prognosis. Methods The immunohistochemical （IHC） assay was performed to detect the expression of CapG protein in 125 specimens of gastric cancer （ study group） and 30 specimens of matched adjacent normal gastric tissues （ control group）. No patients had received chemotherapy or radiotherapy before surgery. Immunostaining was scored semiquantitatively by two independent observers who were blinded to the patients ＇ outcome and other clinicopathological parameters. The correlation of the expression of CapG with elinicopathological characteristics and prognosis was analyzed. Results Cytoplasmic and nuclear CapG protein staining was detected in all gastric cancer tissues. CapG protein expression was absent or low in matched adjacent normal gastric tissues but significantly higher in gastric cancer tissues. Expression of CapG in the tumor was not significantly associated with gender, age, tumor size, tumor location （P 〉 0. 05）. However, elevated CapG expression was strongly correlated with the depth of invasion （P =0. 044）, lymph node metastasis （P =0. 026）, distant metastasis （P =0. 001 ）, and AJCC stage （P = 0. 012）. Significant poorer overall survival was observed in the high CapG expression group compared with that of the low CapG expression group （P 〈 0. 001 ）. Multivariate analysis showed overall survival correlated with the patients＇ tumor location, lymph node metastasis, distant metastasis, AJCC stage and the expression of CapG protein. Conclusion CapG is highly expressed in gastric cancer. Tumors with CapG up-regulation tend to have poorer prognosis, suggesting overexpression of CapG may contribute to the prediction of poorer prognosis and may be a potential therapeutic target for gastric cancer.

关 键 词：胃肿瘤 CapG 预后 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]