一种主要果菜类蔬菜杂种纯度检测的DNA快速提取方法  

A Rapid DNA Extraction Method for Purity Identification of Fruit-vegetables Hybrids

作　　者：高莉洁[1] 周腾夏 葛海燕[1] 路凤琴[1] 刘杨[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《分子植物育种》2016年第3期667-672,共6页Molecular Plant Breeding

基　　金：上海市科委科技支撑项目(13391901201);上海市种业发展项目(沪农科攻字(2012)第2-1)共同资助

摘　　要：本研究提出一种快速提取番茄、茄子、黄瓜DNA的新方法,旨在解决常规蔬菜进行杂种纯度检测时提取DNA特别耗时费力这一难点。实验结果表明,用该方法提取DNA,操作简单、快速、高效、耗费低,不使用高毒试剂,对操作者身体危害很小。同时,电泳检测结果也表明,该方法获得的DNA的质量完全能满足PCR反应的需求,用该种方法获得的DNA可以用于主要果菜类蔬菜杂种纯度检测工作,并且以此可以大大提高主要果菜类蔬菜种子杂种纯度检测工作的效率。In this study,we proposed a novel method for rapidly extracting DNA of tomato,eggplant and cucumber,in order to solve the problem of time-consuming in extracting DNA for hybrid purity detection of common vegetables.Experimental results showed that this DNA extraction method has many advantages such as easy,rapid,efficient and low cost.Using low toxic reagents would be less harmful to the operators.Meanwhile,the results of electrophoresis detection indicated that the quality of DNA extracted in this method can completely meet the criterion of PCR and the product can be used for purity testing of vegetable hybrid.This study can greatly improve the efficiency on identifying the purity of hybrids.

关 键 词：DNA 快速提取法 杂种纯度鉴定 

分 类 号：S63[农业科学—蔬菜学]