洛伐他汀对抗人神经母细胞瘤SH-SY5Y细胞β-淀粉样蛋白的非胆固醇依赖性保护作用  

Protective Effect of Lovastatin on SH-SY5Y Cells against β-amyloid Peptide in Non Cholesterol Dependence Way

作　　者：赵亮[1,2] 官志忠[2,3,4] 

机构地区：[1]贵州医科大学微生物学教研室,贵州贵阳550025 [2]贵州医科大学附院病理科,贵州贵阳550004 [3]贵州医科大学地方病与少数民族性疾病教育部重点实验室,贵州贵阳550004 [4]贵州省医学分子生物学重点实验室贵州医科大学,贵州贵阳550004

出　　处：《贵州医科大学学报》2016年第12期1370-1375,共6页Journal of Guizhou Medical University

基　　金：国家自然科学基金(81260173);教育部"长江学者和创新团队发展计划资助"(IRT13058);贵州省科技计划[黔科合重大专项字(2014)6008号];贵州省创新计划项目[黔教合协同创新中心(2014)06]

摘　　要：目的:观察洛伐他汀对过表达APP670/671的人神经母细胞瘤SH-SY5Y细胞活性及β-淀粉样蛋白(Aβ)二聚体合成的影响。方法:转染并鉴定过表达APP670/671的SH-SY5Y细胞,筛选无细胞毒性、不影响细胞胆固醇水平浓度的洛伐他汀处理SH-SY5Y细胞24 h,采用CCK-8试验检测细胞的存活率,蛋白印迹法检测Aβ二聚体的蛋白表达水平。结果:过表达APP670/671的SH-SY5Y细胞中APP的蛋白及mRNA表达水平均明显高于野生型细胞;用0.1μmol/L的洛伐他汀处理SH-SY5Y细胞,未见明显细胞毒性作用,胆固醇水平亦未见明显改变;0.1μmol/L洛伐他汀预处理SH-SY5Y细胞24 h,能提高细胞存活率,减少Aβ二聚体的分泌。结论:洛伐他汀具有神经保护作用,可能与减少毒性Aβ的合成有关。Objective： To observe the effect of lovastatin neuroblastoma SH-SYSY cell activity and the synthesis Methods： The human APP670/671 gene was transfected on APP670/671 gene over-expressed human of dimer of beta amyloid protein （Aβ）. into the SH-SYSY cells and identified. The best concentration of lovastatin which had no cell toxicity and did not affect the cell cholesterol concen- tration was screened, which treated SH-SY5Y cells for 24 h. Then, CCK-8 test was used to detect the cell survival rate, and Western blot was adopted to detect the protein expression level of Aβ dimer. Results： The protein and mRNA levels of APP in SH-SYSY/APPSWE cells were obviously higher than the SI-I-SYSY wild type cells. No toxic effect and no changes in cholestetol level were observed in the cells treated with 0. 1 μmol/L lovastatin for 24 h. 0. 1 μmol/1 lovastatin pretreatment of SH-SYSY cells with 24 h could improve the survival rate of cells and decreased Aβ dimer secretion. Conclusions： Lovastatin has a neuroprotective effect, which may be related to reducing the toxic Aβ dimer synthesis.

关 键 词：洛伐他汀 Β-淀粉样蛋白 淀粉样前体蛋白 阿尔兹海默病 

分 类 号：R749.16[医药卫生—神经病学与精神病学]