Kruppel样因子17抑制肾癌786-0细胞迁移和侵袭及机制  

The mechanism of Kruppel - like factor 17 inhibit migration and invasion ability of renal cell carci- noma 786-0 c

作　　者：严奉奇 王秦豪[2] 茹懿[2] 李霞[2] 武国军[1] 

机构地区：[1]第四军医大学西京医院泌尿外科,西安710032 [2]第四军医大学生物化学与分子生物学教研室,西安710032

出　　处：《中华实验外科杂志》2016年第12期2672-2674,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金（81272812）

摘　　要：目的探讨Kruppe1样因子17（KLF17）是否通过调控上皮-间充质转化（EMT）抑制786-0细胞的迁移侵袭能力。方法设计并合成特异性小干扰RNA（siRNA）下调肾癌细胞株786-0中KLF17的表达，并利用划痕实验及Transwell实验检测其迁移侵袭能力的变化，通过Westernblot检测EMT相关分子表达水平变化。结果siRNA能有效下调肾癌786-0细胞株中KLFl7的蛋白及mRNA的表达量。细胞划痕实验结果显示下调KLF17表达后，细胞迁移能力增强（P〈0．01）；Transwell实验中阴性对照组穿膜细胞数为（18．16±2．21）个，而siRNA1组为（29．03±2．48）个（P〈0．01），siRNA2组为（28．93±3．00）个（P〈0．01）。KLF17-siRNA介导的基因沉默下调了上皮细胞表面标记E-钙黏蛋白（E-cadberin）、β-连环蛋白（β-catenin）的表达，上调了间充质干细胞表面标记N-钙黏蛋白（N-cadherin）的表达。结论KLF17可通过调节EMT的进程影响肾癌细胞的迁移侵袭能力。Objective To investigate whether Kruppel -like factor 17 （KLF17） could inhibit the migration and invasion ability of 786 - 0 cells by regulating the epithelial - mesenchymal transition （EMT）. Methods Design and synthesize the specific small interfering RNA （siRNA） that can down - regulate the expression of KLF17 in 786 - 0 cells. SiRNAs were transfected into 786 - 0 cells and the effect was detected by Western blotting and real - time quantitative polymerase chain reaction （ Real - time PER）. Migration and invasion ability were tested by the wound scratch assay and the Transwell chamber assay. The expression levels of EMT related molecules J3 - catenin, E - cadherin and N - cadherin were detected by Western blotting. Results SiRNA can effectively down - regulate the expression of KLF17 in 786 - 0 cells （ P 〈 0. 01 ）. Down - regulation of KLF17 expression can improve the cell migration and inva- sion capacity. The cell number in negative control group, siRNA1 group, siRNA2 group was 18. 16 ± 2. 21, 29. 03 ± 2.48 and 28.93 ± 3.00 respectively （ P 〈 0.01 ）. In addition, the expressions of E - cadherin and β - catenin were decreased, while the expression of N - cadherin was increased in siRNA groups. Conclusion KLF17 may regulate EMT to inhibit migration and invasion in 786 -0 cells. KLF17 may be associated with the development and metastatic in renal cell carcinoma.

关 键 词：肾癌 Krtippel样因子17 迁移 侵袭 上皮一间充质转化 

分 类 号：R737.11[医药卫生—肿瘤]