细胞因子诱导杀伤细胞联合p38抑制剂对食管癌EC109细胞的作用  

Effect of cytokine induced killer cells combined with p38 inhibitors on esophageal carcinoma cells EC109

作　　者：霍书华[1] 钟根深[2] 许芝山[2] 李静雅[2] 赵宝生[1] 李汉臣[1] HUO Shu-hua ZHONG Gen-shen XU Zhi-shan LI Jing-ya ZHAO Bao-sheng LI Han-chen(Department of Thoracic Surgery, the First Affiliated Hospital of Xinxiang Medical University, Weihui 453100 ,Henan Prov- ince, China The First Affiliated Hospital of Xinxiang Medical University, Henan Provincal Institute of Neurology, Weihui 453100 , Henan Province, China)

机构地区：[1]新乡医学院第一附属医院胸外科,河南卫辉453100 [2]新乡医学院第一附属医院河南省神经病学研究所,河南卫辉453100

出　　处：《新乡医学院学报》2016年第2期87-90,共4页Journal of Xinxiang Medical University

基　　金：国家自然科学基金青年科学基金资助项目(编号:81281765);河南省高等学校重点科研项目(编号:15A320063)

摘　　要：目的探讨细胞因子诱导杀伤(CIK)细胞联合p38抑制剂对食管癌EC109细胞的作用。方法常规分离健康人外周血单个核细胞,并诱导为CIK细胞,流式细胞仪检测其免疫表型;将食管癌EC109细胞分为空白对照组(不做处理)、p38抑制剂组(加入p38抑制剂)、CIK组(加入培养14 d的CIK细胞)和联合组(加入p38抑制剂联合培养14 d的CIK细胞)。乳酸脱氢酶释放法检测各组食管癌EC109细胞的存活率;流式细胞仪检测各组食管癌EC109细胞的周期阻滞率和凋亡率。结果空白对照组、p38抑制剂组、CIK组及联合组EC109细胞存活率分别为100.00%、75.00%、50.00%和25.00%,对食管癌细胞G1期的阻滞率分别为31.46%、42.04%、50.16%和72.02%,食管癌EC109细胞凋亡率分别为7.15%、19.31%、42.15%和67.17%,其中p38抑制剂组、CIK组及联合组EC109细胞存活率、对G1期的阻滞率、细胞凋亡率均显著高于空白对照组(P<0.05),联合组以上指标均显著高于p38抑制剂组和CIK组(P<0.05)。结论 CIK联合p38抑制剂对食管癌EC109细胞具有较强的杀伤作用。Objective To investigate the effect of cytokine induced killer（ CIK） cells combined with p38 inhibitors on esophageal carcinoma cell EC109. Methods Healthy human peripheral blood mononuclear cells were isolated and induced to CIK cells,and flow cytometry instrument was used to detect the immune phenotype. The esophageal carcinoma cell EC109 were divided into 4 groups： blank control group（ without any treatment）,p38 inhibitor group（ adding p38 inhibitor）,CIK group（ adding CIK cells cultivated for 14 days） and combined group（ adding p38 inhibitor and CIK cells）. Lactate dehydrogenase release method was used to detect the survival rate of esophageal carcinoma cells EC109 in each group. The phase arresting rate and apoptosis rate of esophageal carcinoma cells EC109 in each group was detected by flow cytometry. Results In blank control group,p38 inhibitor group,CIK group and combined group,the survival rate of esophageal carcinoma cells EC109 was100. 00%,75. 00%,50. 00% and 25. 00% respectively,the G1 phase arresting rate of esophageal carcinoma cells EC109 was31. 46%,42. 04%,50. 16% and 72. 02% and the apoptosis rate of them was 7. 15%,19. 31%,42. 15% and 67. 17% respectively. The survival rate,phase arresting rate and apoptosis rate of esophageal carcinoma cells EC109 in p38 inhibitor group,CIK group and combined group were significantly higher than those in blank control group（ P〈0. 05）,while they were significantly higher in combined group compared with those in p38 inhibitor group and CIK group（ P〈0. 05）. Conclusion Combination of CIK with p38 inhibitors has powerful effect of killing activity on esophageal cancer EC109 cells.

关 键 词：食管癌 细胞因子诱导杀伤细胞 p38抑制剂 流式细胞术 

分 类 号：R731[医药卫生—肿瘤]