大白菜玻利玛细胞质雄性不育系92-09A的形态特征及相关基因的克隆与表达模式分析  

Morphological Characteristics of Pol CMS Line of Chinese Cabbage( Brassica rapa L. ssp. pekinensis) and Cloning and Expression Pattern Analysis of Related Sterility Genes

作　　者：王红霞[1,2] 李洪雷 郝建梅 丁谦[1] 张一卉[1] 王凤德[1] 邓永林 王均邦 何启伟[1] 李景娟[1] 高建伟[1,2] 

机构地区：[1]山东省农业科学院蔬菜花卉研究所/山东省设施蔬菜生物学重点实验室/国家蔬菜改良中心山东分中心,山东济南250100 [2]山东大学农学院,山东济南250100 [3]山东登海种业股份有限公司西由种子分公司/山东省大白菜工程技术研究中心,山东莱州273165 [4]济南市长清区双泉镇农业技术推广站,山东济南250303

出　　处：《山东农业科学》2016年第12期6-15,共10页Shandong Agricultural Sciences

基　　金：山东省现代农业产业技术体系蔬菜创新团队建设项目(SDAIT-02-022-04);烟台市科技计划项目(2014NC045);山东省农业科学院重大成果培育计划项目(2015CGPY09)

摘　　要：玻利玛细胞质雄性不育(Pol CMS)是目前很有利用价值的胞质雄性不育源。在前期的研究中,我们利用柯桂兰提供的大白菜Pol CMS不育源为母本,以大白菜骨干亲本92-09为轮回亲本,培育出大白菜Pol CMS不育系92-09A。本研究对大白菜Pol CMS不育系92-09A及其保持系92-09、恢复系早28的形态特征进行观察,并对相应的不育基因Braraorf224及其恢复基因Brara PPR进行了克隆及生物信息学分析。结果表明,大白菜Pol CMS不育系92-09A的花瓣、萼片和雌雄蕊都比保持系92-09小,不能形成有活力的成熟花粉粒;其保持系和杂种F1(不育系92-09A×恢复系早28)均可以产生有活力的花粉粒。大白菜Pol CMS不育基因Braraorf224及恢复基因Brara PPR与甘蓝型油菜的Pol CMS不育基因及恢复基因同源性很高。qRT-PCR结果显示,Brara PPR在恢复系早28和杂种F1中的表达量均明显高于雄性不育系92-09A。本研究可为进一步揭示大白菜Pol CMS雄性不育机理奠定基础。Polima cytoplasmic male sterile（ Pol CMS） is a very important CMS source in plant breeding.In the previous study,we used Pol CMS line KG-Pol of Chinese cabbage provided by Professor Ke Guilan asfemale parent and core line 92-09 as recurrent parent to breed Pol CMS line 92-09 A of Chinese cabbage.Here,the morphological characteristics of Chinese cabbage Pol CMS line 92-09 A,its parental maintainer line 92-09 and restorer line Zao 28 were investigated,and the Pol CMS gene Braraorf224 and its restorer gene Brara PPR were cloned and analyzed. The results showed that the petals,sepals,stamens and pistils of Chinese cabbage Pol CMS line 92-09 A were all smaller than those of its maintainer line 92-09,and it could not form mature pollen with viability. While the maintainer line 92-09 and the F1 hybrid of Pol CMS line92-09 A × restorer line Zao 28 both had pollen grains with viability. Bioinformatics analysis indicated that the nucleic acids and amino acids of Chinese cabbage Pol CMS gene Braraorf224 and restorer gene Brara PPR were very close to those of Brassica napus. The results of qRT-PCR showed that the expression level of Brara PPR in restorer line Zao 28 and F1 hybrid were both higher than that in Pol CMS line 92-09 A. The research would be helpful to understand the molecular mechanism of Pol CMS of Chinese cabbage in the future.

关 键 词：大白菜 POL CMS 不育基因 恢复基因 

分 类 号：S634.1[农业科学—蔬菜学] Q78[农业科学—园艺学]