P-Cx43在人胶质瘤细胞中的表达及其与肿瘤细胞增殖相关性研究  

Expression of phosphorylation gap junction protein 43 in human glioma and correlation with tumor cell proliferation

作　　者：姬云翔[1] 王业忠[1] 赵冬[1] 董江涛[1] 田卫东[1] 王刚刚[1] 

机构地区：[1]石河子大学医学院第一附属医院神经外科,新疆石河子832000

出　　处：《重庆医学》2016年第36期5091-5094,共4页Chongqing medicine

摘　　要：目的探讨磷酸化缝隙连接蛋白43(P-Cx43)在人脑胶质瘤细胞中的表达水平及其与肿瘤细胞增殖的相关性。方法该科2013年6月至2014年6月手术切除内减压正常脑组织10例作为对照组,同时间段切除人脑胶质瘤标本63例为试验组。应用HE染色、免疫组化法确定胶质瘤性质等级,通过Ki-67的染色反映肿瘤细胞增殖情况。采用蛋白质印迹法(Western blot)技术检测P-Cx43的表达。结果研究发现,在正常脑组织中,P-Cx43的表达较WHOⅠ级胶质瘤中表达略低,但二者差异无统计学意义(P=0.140),其余各组间表达差异有统计学意义(P<0.05)。在各组之间P-Cx43表达差异有统计学意义(P<0.01),呈现逐渐增加的趋势。结论 P-Cx43与肿瘤细胞活性和胶质瘤恶性进展密切相关。Objective To explore the expression of phosphorylation gap junction protein 43（P‐Cx43） level in human gliomas and correlation with tumor cell proliferation .Methods Ten cases of normal brain tissue from June 2013 to June 2014 in Department of Neursurgery were chosen as control group ,63 cases of glioma were resected in the same period of time was experience group .HE staining and immunohistochemistry were used to determine the grade of gliomas ,and the proliferation of tumor cells was determined by Ki‐67 staining .The expression of P‐Cx43 protein was detected by Western blot technique .Results It was found that in normal brain tissue ,the expression of P‐Cx43 was slightly lower than that WHO Ⅰ in gliomas ,but there was no significant difference be‐tween the two groups（P=0 .140） ,there were significant differences among the other groups （P〈0 .05） .There was a significant difference in P‐Cx43 expression between the groups （P〈 0 .01） ,showing a gradually increasing trend .Conclusion The P‐Cx43 protein was closely related to the activity of tumor cells and the malignant progression of gliomas .

关 键 词：细胞增殖 连接蛋白类 细胞系 肿瘤 脑胶质瘤 

分 类 号：R739.41[医药卫生—肿瘤]