EphB4在肝癌组织中的表达及临床意义  

Expression of EphB4 in hepatocellular carcinoma tissues and its clinical significance

作　　者：曹阳[1] 吴念[1] 刘桦[1] 

机构地区：[1]重庆市第五人民医院普外科,400062

出　　处：《重庆医学》2016年第36期5110-5112,5115,共4页Chongqing medicine

摘　　要：目的研究酪氨酸蛋白激酶受体EphB4在肝癌组织中的表达及临床意义,并分析EphB4对肝癌细胞增殖的影响。方法采用逆转录-聚合酶链反应(RT-PCR)和免疫组化技术分析EphB4在60例肝癌组织与相应癌旁组织中的表达差异,分析EphB4在肝癌组织中的表达水平与肝癌患者临床病理学之间的相关性,进行单因素生存分析,MTS实验分析EphB4对肝癌细胞系SK-Hep-1活力的影响。结果 RT-PCR检测结果显示EphB4在肝癌组织中mRNA表达水平(1.39±0.80)。明显高于癌旁组织(0.56±0.33),差异有统计学意义(P<0.01);免疫组化分析发现EphB4在肝癌组织和癌旁组织中的阳性率分别为81.7%和23.3%;EphB4在肝癌组织中的表达与AFP水平、肿瘤大小、TNM分期相关(P<0.05);EphB4蛋白表达阳性和阴性的肝癌患者3年生存率分别为22.5%和54.5%,差异有统计学意义(P<0.05);过表达EphB4显著提高肝癌细胞增殖能力(P<0.05)。结论 EphB4在肝癌组织中表达显著上调,与肝癌进展和预后相关;并且EphB4能促进肝癌细胞增殖,其可作为肝癌进展的标志物之一。Objective To study the expression of Ephrin‐B4 receptor （EphB4） in hepatocellular carcinoma （HCC） and its clinical significance ,and to analyze the effect of EphB4 on the proliferation of HCC cells .Methods The expression level of EphB4 in HCC tissues and matched paracancerous liver tissues of 60 cases of HCC patients was assessed by reverse transcriptase‐polymer‐ase chain reaction （RT‐PCR） and immunohistochemistry .The correlation between the expression of EphB4 in HCC tissues and clin‐ical pathologic parameters was analyzed by chi‐square test .Univariate survival analysis was carried out by Kaplan‐Meier Log‐rank test .The effect of EphB4 on the viability of HCC cells was furtherly analyzed by MTS .Results The results of RT‐PCR showed that the mRNA level of EphB4 in HCC tissues （1 .39 ± 0 .80） was significantly higher than in matched paracancerous liver tissues （0 .56 ± 0 .33） ,the difference was statistically significant （P〈 0 .01） .Data from immunohistochemistry showed that the positive rates of EphB4 protein in HCC tissues and matched paracancerous liver tissues were 81 .7% and 23 .3% ,respectively .Moreover ,the expression of EphB4 in HCC tissues was relevant to AFP level ,tumor size and TNM stage（P〈0 .05） .The three‐year survival rate of HCC patients with positive expression of EphB4 protein was 22 .5% ,and that of HCC patients with negative expression of EphB4 protein was 54 .5% ,the difference was statistically significant （P〈0 .05） .Overexpression of EphB4 significantly enhanced the ability of hepatocellular carcinoma cell proliferation （P〈0 .05） .Conclusion EphB4 expression was significantly up‐regulated in HCC ,which was associated with HCC progression and prognosis ,and EphB4 could promote the proliferation of HCC cells ,which could be used as a marker of HCC progression .

关 键 词：肝肿瘤 EPHB4 临床意义 

分 类 号：R735.7[医药卫生—肿瘤]