Krüppel-like factor 4在小鼠舌黏膜癌变过程中的表达  

Expression of Krüppel-like factor 4 in 4NQO-induced mouse tongue carcinogenesis model

作　　者：宿颖[1] 刘曼[1] 孙丽丽[1] 孙正[1] 张辛燕[1] 

机构地区：[1]首都医科大学口腔医学院口腔医学研究所,北京100050

出　　处：《北京口腔医学》2016年第6期305-308,共4页Beijing Journal of Stomatology

基　　金：国家自然科学基金(81272982);北京市自然科学基金(7162073)

摘　　要：目的检测Krüppel-like factor 4(KLF4)在小鼠舌黏膜癌变模型中的表达,探讨KLF4与口腔黏膜癌变的关系。方法选用C57BL/6J小鼠75只,其中阴性对照组15只,饮用纯净水;实验组小鼠60只,饮用4-硝基喹啉-1-氧化物(4NQO)水溶液(50μg/ml)。实验组分别在第8、12、16、24周末各处死15只小鼠,取舌组织,观察并记录标本的大体损害,将其固定在10%中性福尔马林溶液中,用于HE染色及KLF4免疫组织化学染色。结果在4NQO的诱导下,成功构建了小鼠舌黏膜癌变模型。HE染色结果显示,随着4NQO处理时间的延长,小鼠舌黏膜病变逐步加重,呈现出从单纯增生、异常增生到癌的变化。免疫组化结果显示,KLF4在口腔癌变过程中呈现动态变化,与正常口腔黏膜上皮相比,KLF4在单纯增生、异常增生上皮中的表达没有明显变化,但在鳞癌中,KLF4的表达量明显下降,具有显著性差异(P<0.01)。结论 KLF4可能在口腔黏膜上皮癌变过程中发挥抑癌基因的作用。Objective To examine the expression of KLF4 in 4NQO-indueed mouse tongue carcinogenesis model. Methods Seventy-five C57BL/6J mice were randomly divided into control group and four experimental groups. The control group received no treatment, while the 4 experimental groups were given 4NQO （50μg/ml）in drinking water for 8, 12 and 16 weeks respectively. The mice were sacrificed at 8th, 12th, 16th and 24th week respectively. The samples were stained with HE and the expression of KLF4 was detected by immunohistoehemistry. Results C57BL/6J mouse tongue carcinogenesis model was successfully established by 4NQO. HE staining showed that with the extension of 4NQO treatment time, the mouse tongue mucosa changed from epithelium hyperplasia to dysplasia and squamous cell carcinoma. The expression of KLF4 was not significantly different between the normal mucosa and hyperplasia and dysplasia. However, the expression of KLF4 markedly decreased in the severe dysplasia and squamous cell carcinoma compared with the control group （P 〈0.01 ）. Conclusion KLF4 may play a role in oral carcinogenesis as a tumor suppressor gene.

关 键 词：KLF4 口腔鳞癌 动物模型 

分 类 号：R739.8[医药卫生—肿瘤]