小鼠切牙干槽症模型建立的实验研究  

Establishment of the animal model of dry socket in mouse'incisors

作　　者：张宁[1] 常世民[1] 张瑞[1] 娜荷雅[1] 王克迪[2] 黄晓峰[1] 

机构地区：[1]首都医科大学附属北京友谊医院口腔科,北京100050 [2]首都医科大学临床检验科,北京100050

出　　处：《北京口腔医学》2016年第6期309-311,共3页Beijing Journal of Stomatology

基　　金：北京市自然基金(7162053)

摘　　要：目的建立干槽症的小鼠模型。方法取10只小鼠,随机分为实验组和对照组。每只小鼠腹腔注射1.5%戊巴比妥麻醉后,拔除上颌中切牙。实验组拔牙窝行肾上腺素止血,植入金黄色葡萄球菌标准菌种,对照组拔除上颌中切牙后无特殊处理。4天后观察两组拔牙窝情况并处死小鼠,取上颌骨进行组织切片、HE染色,观察两组拔牙窝的结构变化。结果拔牙4天后,实验组拔牙窝见大量脓性分泌物,周围黏膜红肿,对照组拔牙窝粘膜呈粉红色,创口较拔牙后明显缩小,无明显分泌物。观察组织学HE染色切片发现,实验组拔牙窝可见大量炎性细胞及少量破骨细胞,对照组的拔牙窝内有新生结缔组织形成。结论使用肾上腺素和金黄色葡萄球菌标准菌种可以成功地建立小鼠切牙拔牙窝的干槽症模型。Objective To establish the animal model of dry socket in mouse. Methods Ten C57BL/6J mice were randomly divided into two groups. The right upper incisors of the mouse were pulled out. The experimental group received adrenaline solution followed by Staphylococcus aureus. The control group was not treated. Four days after extraction, the tooth extraction sockets were observed and all mice were sacrificed for histological examination. Results The experimental group showed pus and gingival edema, while the control group showed pink gingival without any secretions 4 days after extraction. The experimental group exhibited a large number of neutrophils and a small number of osteoclasts, and the control group showed a large number of blood clots, connective tissue in the socket. Conclusion The animal model of dry socket in mouse was successfully established.

关 键 词：干槽症 小鼠 模型 

分 类 号：R782.13[医药卫生—口腔医学] R-332[医药卫生—临床医学]