EGFR抑制剂西妥昔对舌鳞癌多药耐药细胞Tca8113/CBP细胞内阿霉素浓度的影响  

Effect of anti-EGFR agents Cetuximab on intracellular drug concentration in tongue squamous cell carcinoma drug-resistance cell Tca8113/CBP

作　　者：刘得玺[1] 毛敏[1] 窦志茜 陈永吉[1] 曾宪涛[1] 甘丽[1] 夏凌云[1] 罗志晓[1] 冷卫东[1] 

机构地区：[1]湖北医药学院附属十堰市太和医院口腔医学中心,十堰442000 [2]湖北医药学院附属十堰市太和医院产科,十堰442000

出　　处：《北京口腔医学》2016年第6期312-314,共3页Beijing Journal of Stomatology

基　　金：湖北省教育厅科研计划项目(D20122405)

摘　　要：目的探讨表皮生长因子受体(EGFR)抑制剂西妥昔在舌鳞癌多药耐药中的作用。方法 Western blot方法检测舌癌细胞系Tca8113及多药耐药细胞系Tca8113/CBP EGFR的表达,流式细胞术检测EGFR受到抑制后舌癌多药耐药细胞Tca8113/CBP细胞内阿霉素浓度的影响。结果细胞系Tca8113和Tca8113/CBP均有EGFR的表达(P>0.05)。EGFR受到抑制后,Tca8113/CBP细胞内阿霉素浓度升高(P<0.05)。结论 EGFR抑制剂西妥昔可使舌癌多药耐药细胞细胞内药物浓度升高。Objective To investigate the effect of anti-EGFR agents Cetuximab on multidrug resistance （MDR） tongue squamous cell carcinoma cell line TcaS113/CBP. Methods Western blot assay was applied to detect EGFR expression in tongue squamous cell carcinoma cell line TcaS113 and its MDR cell line Tca8113/CBP. The concentration of intracellular drug adriamycin in cell line Tca8113/CBP after application of Cetuximab was measured by flow cytometry. Results EGFR in cell lineTcaSll3 and its MDR cell line TcaSll3/CBP was expressed （P 〉0.05）. After the use of anti- EGFR agents Cetuximab, the intracellular drug concentration of adriamycin was significantly increased in MDR cell line Tca8113/CBP （P 〈 0. 05 ）. Conclusion The anti--EGFR agent Cetuximab could increase the intracellular drug concentration in MDR cell line TcaSll3/CBP.

关 键 词：EGFR 西妥昔 舌癌多药耐药 细胞内药物浓度 Tca08113/CBP细胞 

分 类 号：R739.86[医药卫生—肿瘤]