大鼠侧脑室注射Apelin-13对急性痛的调节作用及机制  

The modulation and mechanism of Apelin-13 in an acute pain model

作　　者：徐震[1] 王庆尧 王贤斌[1] 武菲[1] 

机构地区：[1]济宁医学院神经生物学研究所,272067

出　　处：《中华诊断学电子杂志》2016年第4期279-283,共5页Chinese Journal of Diagnostics(Electronic Edition)

基　　金：2016年地方高校国家级大学生创新创业训练计划项目[201610443048];济宁医学院青年基金[JYQ14KJ19];济宁医学院大学生创新训练计划项目[CX2015005];2015年度济宁市医药卫生计划项目[79];济宁医学院2015年度大学生科研课题

摘　　要：目的探讨Apelin-13对大鼠急性痛的调制作用及可能机制。方法简单随机抽样将50只大鼠分为生理盐水组、Apelin-13 0.05μg/g组、Apelin-13 0.1μg/g组、Apelin-13 0.5μg/g组及吗啡组(阳性对照组),每组10只,采用辐射热甩尾法连续监测60 min内大鼠痛阈的变化;同样采用简单随机抽样将40只大鼠分为生理盐水组、Apelin-13 0.5μg/g组、酪蛋白激酶2(CK2)抑制剂四溴苯三唑(TBB)组及Apelin-13+TBB组,每组10只,监测其痛阈的变化。Western Blot法检测海马内CK2表达量及N-甲基-D-天冬氨酸(NMDA)受体NR2B亚单位(P-NR2B S1480)磷酸化水平,研究Apelin-13对急性痛调制作用可能的机制。结果 Apelin-13可显著升高急性痛痛阈,0.5μg/g组给药10 min时甩尾潜伏期变化率(TWL%)达峰值[(50.03±2.71)%],与生理盐水组比较差异有统计学意义(q=18.054,P=0.003),并且此效应呈剂量和时间依赖(F_(Group)=47.729,P=0.000;F_(Time)=205.301,P=0.000)。同时给予CK2抑制剂TBB 10 min时TWL%降至(17.75±1.67)%,低于Apelin-13组,差异有统计学意义(q=6.976,P=0.005)。Western Blot结果显示TBB可明显减弱Apelin-13的作用,CK2α表达量、NR2B亚单位磷酸化水平均下降[(26.92±4.38)%,(39.90±7.40)%],与Apelin-13组[(41.60±6.65)%,(70.83±3.52)%]比较差异有统计学意义(q=5.246,P=0.010;q=9.757,P=0.010)。结论 Apelin-13可能通过CK2促进NMDA受体NR2B亚单位磷酸化,进而在热甩尾急性痛模型中起镇痛作用。Objective To explore the modulation and mechanism of Apelin-13 in an acute pain model. Methods The pain thresholds were continuously monitored in 60 min after intracerebroventricular （ICV） administration of Apelin-13 （0.05,0.1 and 0.5μg/g） and casein kinase 2（CK2） inhibitor 4,5,6, 7-tetrabromoben：zotriazole （ TBB ） by radiant heat tail-flick test. Then the expression of CK2 and phosphorylation of NR2B S1480 in hippocampus were detected by Western Blot. Results ICV injection of Apelin-13 induced a dose and time-dependent antinociceptive effect. This effect was significantly antagonized by CK2 inhibitor TBB.Western Blot showed that Apelin-13 could significantly promote the expression of CK2 and the phosphorylation of NR2B S1480,which decreased obviously by TBB.Conclusion Apelin-13 plays an antinociceptive role in the acute pain model of radiant heat tail-flick test via modulating CK2 expression and the phosphorylation of NR2B S1480.

关 键 词：APELIN 急性痛 镇痛 酪蛋白激酶Ⅱ NR2B亚单位 

分 类 号：R741[医药卫生—神经病学与精神病学]