圈养林麝乳突类圆线虫的分子鉴定  

Molecular identification of Strongyloides papillosus from captive Moschus berezovskii

作　　者：林海[1] 郑从军 程建国[3] 蔡永华[3] 王洪永 颜其贵[1] 杨光友[1] 

机构地区：[1]四川农业大学动物医学院,四川成都611130 [2]宝兴县林业局,四川雅安625700 [3]四川养麝研究所,四川成都611830 [4]四川逢春制药有限公司,四川成都610081

出　　处：《中国兽医科学》2016年第12期1550-1555,共6页Chinese Veterinary Science

基　　金：四川省科技支撑计划项目(2014SZ0132;2014Zc2733)

摘　　要：为了对林麝粪样中的类圆属线虫的虫卵进行虫种鉴定,提取了四川和陕西地区林麝粪样中类圆属线虫的虫卵DNA,采用PCR技术扩增18S r RNA基因序列并进行序列分析。结果显示,扩增出的片段大小均为362 bp,21个来自林麝粪样中的类圆属线虫虫卵样品的18S r RNA基因序列完全相同,经序列BLAST比对,与Gen Bank中的多株乳突类圆线虫的序列相似性为98.5%~100%。本研究结果表明,寄生于圈养林麝体内的类圆属线虫均为乳突类圆线虫。To identify the Strongyloides sp.from fecal samples of captive Moschus berezovskii collected in Sichuan and Shaanxi Province of China,the DNA was extracted from eggs of Strongyloides sp.,the 18 S r RNA was amplified by PCR and the amplicons were sequenced and analyzed.The 18 S r RNA sequences obtained from 21 specimens shared the same fragments of 362 bp,which had a similarity from 98.5% to100% to several Strongyloides papillosus isolates in Gen Bank.BLAST alignment indicated that all the Strongyloides sp.from M. berezovskii were S.papillosus.

关 键 词：林麝 乳突类圆线虫 分子鉴定 PCR 18S RRNA 

分 类 号：S852.731[农业科学—基础兽医学]