银杏二萜内酯对缺血/再灌注大鼠脑组织中神经递质的影响  

Effects of diterpene ginkgolides meglumine injection on neurotransmitters in cerebral ischemia-reperfusion injury rats

作　　者：张雯[1] 宋俊科[1] 何国荣[1] 张雪[1] 周启蒙[1] 王振中[2] 萧伟[2] 肖智勇[3] 周文霞[3] 杜冠华[1] 

机构地区：[1]中国医学科学院北京协和医学院药物研究所、北京市药物靶点研究与新药筛选重点实验室,北京100050 [2]中药制药过程新技术国家重点实验室、江苏康缘药业股份有限公司,江苏连云港222001 [3]军事医学科学院毒物药物研究所、抗毒药物与毒理学国家重点实验室,北京100850

出　　处：《中国药理学通报》2016年第12期1648-1656,共9页Chinese Pharmacological Bulletin

基　　金：国家科技部“重大新药创制”科技重大专项资助项目(No2013ZX09508104,2013ZX09402203);抗毒药物与毒理学国家重点实验室开放课题(No TMC201510);国家自然科学青年基金项目(No 81603100);北京协和医学院研究生创新基金项目(No 2015-1007-07)

摘　　要：目的探讨银杏二萜内酯葡胺注射液对缺血/再灌注大鼠脑内氨基酸类和单胺类神经递质的影响。方法采用线栓法制备大鼠脑缺血/再灌注模型,缺血1.5 h,再灌注24h,尾静脉给予不同剂量银杏二萜内酯葡胺注射液后,用高效液相色谱串联电化学检测器(HPLC-ECD)测定脑内氨基酸类和单胺类神经递质含量。结果银杏二萜内酯葡胺注射液可以抑制缺血/再灌注导致的脑内天冬氨酸、谷氨酸、甘氨酸和γ-氨基丁酸的升高;降低缺血脑区皮层和海马内去甲肾上腺素、二羟苯乙酸、5-羟色胺、5-羟吲哚乙酸的浓度,抑制皮层内肾上腺素的减少。结论银杏二萜内酯葡胺注射液可以通过调节脑内氨基酸类和单胺类神经递质的水平,对大鼠脑缺血/再灌注引起的损伤发挥治疗作用。Aim To investigate the effects of diterpene ginkgolides meglumine injection（ DGMI） on amino acids and monoamine neurotransmitters in rats with cerebral ischemia / reperfusion injury. Methods Intraluminal suture was applied to establish middle cerebral artery occlusion（ MCAO / R） model with ischemia for 1. 5 h and reperfusion for 24 h. After the administration of DGMI（ i. v.）,the levels of amino acid and monoamine neurotransmitters in brain tissue were detected through HPLC-ECD. Results DGMI down-regulated the concentrations of aspartic acid, glutamic acid,glycine and γ-aminobutyric acid which were increased in MCAO / R group. DGMI also reduced the levels of norepinephrine epinephrine,glyoxylic acid,serotonin and 5-HIAA in cortex and hippocampus,and increased adrenaline content compared to the model group. Conclusion DGMI exhibits a protective role in rats with cerebral ischemia / reperfusion injury through regulating amino acids and monoamine neurotransmitters.

关 键 词：银杏二萜内酯葡胺注射液 脑缺血/再灌注 氨基酸类递质 单胺类递质 HPLC-ECD 代谢组学 

分 类 号：R-332[医药卫生] R282.71