16个X-STR基因座在河南汉族人群中的法医学应用评估  

Forensic Application of 16 X-STR Loci in Henan Han Population

作　　者：刘亚举 郭利红[2] 岳俊涛 石美森[3] 

机构地区：[1]许昌市公安局刑事科学技术研究所,河南许昌461000 [2]河南省公安厅刑事科学技术研究所,河南郑州450003 [3]中国政法大学证据科学教育部重点实验室,北京100088

出　　处：《法医学杂志》2016年第6期420-423,427,共5页Journal of Forensic Medicine

摘　　要：目的调查16个X-STR基因座在河南汉族人群中的遗传学数据,评估其法医学应用价值。方法应用Goldeneye^(TM)DNA身份鉴定系统17X试剂盒,对河南地区326名汉族无关个体DNA进行PCR扩增,3130xl型遗传分析仪电泳分析,Gene Mapper~ID-X软件分析等位基因片段大小。统计分析16个X-STR基因座的频率数据和群体遗传学参数,并与其他地区已有人群数据进行比较。结果在所检测的16个X-STR基因座中,DXS6800具有中度多态性,其余15个基因座均具有高度多态性。这16个X-STR基因座在女性群体的累积个人识别率为0.999 999 999 999 992,在男性群体的累积个人识别率为0.999 999 996 577 712,在三联体中的累积非父排除率为0.999 999 971,在二联体中的累积非父排除率为0.999 992 574。结论这16个X-STR基因座达到了法医物证学应用要求,尤其对特殊的亲权鉴定案件具有重要的应用价值。Objective To investigate the genetic data of 16 X-STR loci in Henan Han population and to assess the application value in forensic science. Methods The DNA of 326 unrelated individuals in Henan Han population were amplified using Goldeneye？ DNA identification system 17X kit, and the PCR products were analyzed by electrophoresis through 3130x1 genetic analyzer. The fragment sizes of alleles were analyzed subsequently by GeneMapper？ ID-X. Allele frequencies and population genetics pa-rameters of 16 X-STR loci were analyzed statistically and compared with the available data of other Han populations from different regions. Results Among the 16 X-STR loci, DXS6800 were found to be moderately polymorphic and the other 15 X-STR loci were highly polymorphic. The cumulative discrimi-nation power in females and males were 0.999 999 999 999 992 and 0.999 999 996 577 712, respectively. The combined power of exclusion in trios and in duos were 0.999 999 971 and 0.999 992 574, respectively. Conclusion The 16 X-STR loci meet the application requires of forensic genetics, especially for testing the special paternity cases.

关 键 词：法医遗传学 多态现象 遗传 X-STR 河南 汉族 

分 类 号：R394[医药卫生—医学遗传学]