两株硫酸盐还原菌的培养条件研究  

Study on the Culture Conditions of Two Sulfate-Reducing Bacterium Strains

作　　者：杜刚[1] 张广求[1] 胡秋月[2] 郭沨 杨海英[2] DU Gang ZHANG Guang-qiu HU Qiu-yue GUO Feng YANG Hai-ying(Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission ＆ Ministry ofEducation, Kunming 650500, China College of Chemistry and Environment, Yunnan Minzu University, Kunming 650500, China The China Hydropower Consulting Group Corporation, Beijing 100011, China)

机构地区：[1]云南民族大学民族药资源化学国家民族事务委员会-教育部重点实验室,昆明650500 [2]云南民族大学化学与环境学院,昆明650500 [3]中国水电顾问集团公司,北京100011

出　　处：《四川农业大学学报》2016年第4期483-487,共5页Journal of Sichuan Agricultural University

基　　金：国家自然科学基金资助项目(21462051);云南中烟工业有限责任公司项目(2015539200340277);云南省教育厅科学研究基金重点项目(2015Z111);云南民族大学民族药资源化学国家民委-教育部重点实验室开放基金项目(MZY1401)

摘　　要：【目的】研究两株SRB菌生长繁殖的影响因素,进行SRB菌剂的初步试制。【方法】采用CFU法进行菌株密度计数,考察不同p H、培养温度、碳源、培养基等条件对两株SRB菌株生长的影响,并对SRB4菌株进行了菌剂的初步试制。【结果】菌株SRB4的最适培养条件:温度为38℃;起始p H为8.0;培养基是以柠檬酸钠为碳源的改良Postgate培养基。菌株SRB3的最适培养条件:温度为36℃;起始p H为7.0;培养基是以玉米芯水提液为碳源的Postgate培养基。经菌剂初步试制,SRB4菌株在发酵48 h后菌株密度保持在1012 CFU/m L以上。【结论】两株SRB菌在最适培养条件下密度保持在1012 CFU/m L以上,可作为工程应用的备选菌株。[Objective] The main factors of the growth of two Sulfate-Reducing Bacterium （SRB） strains were investigated and preliminary trail of SRB microbial inoculum was carried out. [Methods] The impact of pH, temperature, carbon source and culture media on the growth of SRB were studied by the method of CFU for strain density count, moreover, the trial-produce of SRB4 microbial inoculum was implemented. [Results] The optimum culture conditions for SRB4 were as follows： temperature 38 ℃; pH 8.0; provided Postgate medium with sodium citrate as carbon source. The optimmn culture conditions for SRB3 were as follows： temperature 36 ℃;pH 7.0; Postgate medium with the water extract of corn cob as carbon source. The strain density of SRB4 was maintained above 1012 CFU/mL after 48 h femlentation. [Conclusion] Under the optimal conditions,the strain density of the two SRBs were up to 1012 CFU/ mL, and they could be alternative strains for engineering application.

关 键 词：硫酸盐还原菌 培养条件 菌剂 

分 类 号：X703[环境科学与工程—环境工程]