绿茶提取液纳米Ag的制备及表征  

Preparation mechanism and characterization of monodisperse silver nanoparticles synthesized by green tea leaf extract

作　　者：杨辉[1] 任雁宇 YANG Hui REN Yan-yu(School of Food and Biological Engineering, Shaanxi University of Science Technology, Xi^an 710021, China)

机构地区：[1]陕西科技大学食品与生物工程学院,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2017年第1期124-128,138,共6页Journal of Shaanxi University of Science & Technology

基　　金：陕西科技大学学术带头人团队计划项目(2013XSD19)

摘　　要：采用绿茶提取液制备AgNPs,借助UV-Vis跟踪检测反应液中Ag+的还原和Ag单质的生成过程;采用XRD、TEM对所得AgNPs进行组成、结构和表面形貌进行表征;通过FTIR分析探索AgNPs表面化学作用情况;测定了绿茶提取液还原性,对绿茶提取液制备AgNPs的机理进行了分析;采用抑菌圈试验验证所得AgNPs的抗菌性.结果表明:绿茶提取液制备AgNPs快速、简单,粒径10~15nm,大小分布均匀,结晶度高;并对革兰氏阴性菌和革兰氏阳性菌具有较强的抑菌性能;绿茶萃取液具有很强的还原能力,易将Ag+还原成单质银,而其中所含的生物大分子则起到了分散剂的作用.In this project, green tea extract was used to synthesize AgNPs. UV-Vis was em-ployed to detect and track the reduction of Ag＋ and the forming process of AgNPs. The syn-thesize AgNPs with desirable physical and biological properties was characterized using TEM and XRD. FTIR spectroscopy was used to analysis to determine capping of biomolecule on the AgNPs surface of plant extract. Reduction properties of green tea extract were analyzed to study the mechanism of redox reaction. Antibacterial activity of the AgNPs was deter-mined. The results showed that the biosynthesis method of AgNPs is easy and simple. The size of AgNPs between 1〇 ？15 nm and it takes spherical shapes and good crystallinity, and has strong antibacterial activity against gram positive bacteria and gram negative bacteria. Antioxidant experiment results showed that green tea extract has strong reduction capacity, able to reduct Ag＋ into Ag easily, and the biological macromolecules of the extract have sta-bilizing agent properties.

关 键 词：生物合成法 纳米Ag粒子 绿茶提取液 抑菌性能表征 抗氧化 

分 类 号：TB383.1[一般工业技术—材料科学与工程]