B细胞非霍奇金淋巴瘤患者细胞增殖和凋亡相关指标检测结果分析  

Analysis of Indicators Related with Cell Proliferation and Apoptosis in the Patients with B Cell Non-Hodgkin's Lymphoma

作　　者：尹俊杰[1] 梁波[1] 鲁一[2] 展新荣[1] 

机构地区：[1]河南省新乡市中心医院血液科,河南新乡453000 [2]河南省新乡市中心医院肿瘤内二科,河南新乡453000

出　　处：《中国实验血液学杂志》2016年第6期1771-1775,共5页Journal of Experimental Hematology

摘　　要：目的:分析B细胞非霍奇金淋巴瘤(B cell non-Hodgkin's lymphoma,B-NHL)患者细胞增殖和凋亡相关指标的检测结果。方法:分别检测72例B-NHL患者的经病理诊断证实的病灶组织石蜡包埋标本(实验组)和50例淋巴结反应性增生患者的淋巴结石蜡标本(对照组)的增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)、X连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis,XIAP)和B细胞淋巴瘤/白血病-2基因(B-cell lymphoma/leukemia-2,BCL-2)表达情况,并分析其与病理因素的关系。结果:实验组PCNA阳性细胞百分率及XIAP、BCL-2阳性率均显著高于对照组(P<0.05);Ⅲ-Ⅳ期B-NHL患者PCNA阳性细胞百分率显著高于Ⅰ-Ⅱ期患者(P<0.05);不同病理因素B-NHL患者XIAP和BCL-2阳性率并无明显差异(P>0.05);PCNA低阳性表达组无进展生存期显著长于PCNA高阳性表达组(P<0.05);XIAP^+组与XIAP-组无进展生存期并无显著差异(P>0.05);BCL-2^+组与BCL-2^-组无进展生存期亦无显著差异(P>0.05)。结论:增殖因子PCNA与凋亡因子XIAP、BCL-2均参与了B-NHL的发生,其中PCNA阳性情况对临床分期有明显影响。Objective：To analyze the indicators related with cell proliferation and apoptosis in patients with B cell non-Hodgkin＇s lymphoma（B-NHL）.Methods：Seventy-two cases of B-NHL and 50 cases of reactive hyperplasia of lymph nodes were entrolled in the experimental group and control group respectively.The expression levels of proliferating cell nuclear antigen（PCNA）,X-linked inhibitor of apoptosis（XIAP） and B cell lymphoma leukemia-2（BCL-2） in paraffin-embedded tissue samples of 2 groups were detected by immuno-histochemistry staining,and their ralationship with pathologic factors was analyzed.Results：The positive cell rates of PCNA,XIAP and BCL-2 in experiment group were significantly higher than those in control group（P〈0.05）;the positive cell rate of PCNA in B-NHL patients at Ⅲ- Ⅳ stage was higher than that in B-NHL patients at I- II stage（P〈0.05）,however,the positive cell rates of XIAP and BCL-2 in B-NHL patients with different pathologic factors were not significantly different（P〉0.05）.The progression-free survival（PES） time in PCNA low positive expression group was longer than that in PCNA high positive expression group（P〈0.05）,but the PFS time between B-NHL patients with XIAP positive and negative expression was not significantly different（P〉0.05）;the PFS time also was not significantly different between B-NHL patients with BCL-2positive and negative expression（P〉0.05）.Conclusion：All the PCNA,XIAP and BCL-2 participate in the pathngenesis of B-NHL,among them the positive level of PCNA obviously influences the clinical staging of B-NHL.

关 键 词：B-NHL 细胞增殖 细胞凋亡 PCNA XIAP BCL-2 

分 类 号：R733.1[医药卫生—肿瘤]