肌特异性miRNA在肌细胞分化过程及肌营养不良中的表达  

Expression of muscle-specific miRNAs in myoblast differentiation and muscular dystrophy

作　　者：陈雪燕[1,2] 陈裕庆[1] 刘文文[1] 杨丽[1] 许钰[1] 张文敏[1] 

机构地区：[1]福建医科大学基础医学院病理学系 [2]福建省肿瘤医院病理科

出　　处：《解剖学杂志》2016年第6期649-651,663,F0004,共5页Chinese Journal of Anatomy

基　　金：福建省资助省属高校项目(JK2012017)

摘　　要：目的：观察肌特异性miRNA（miR-1，-133a和-206）在肌细胞增殖和分化过程中的变化，探讨肌特异性miRNA与肌营养不良的关系。方法：培养小鼠成肌细胞C2C12并诱导分化成熟，应用实时荧光定量PCR（q-PCR）分别检测C2C12在增殖期和分化期（1、3、5d）miR-1、-133a、-206的变化；免疫组织化学法筛选dystrophin缺失型营养不良病例（DMD），q-PCR检测miR-1、-133a、-206在DMD肌组织中的表达情况。结果：增殖期C2C12细胞的miR-1、-133a、-206表达量较低，分化期3者表达均升高，并随着成肌细胞分化时间的延长，miRNAs表达显著升高，其中miR1表达升高最明显；筛选的10例DMD标本均存在不同程度的dystrophin蛋白表达减弱或缺失，与非特异性改变肌组织对比，miR-1、-133a、-206表达均升高，其中miR-206在DMD患者肌组织的表达水平显著升高。结论：miR-1、-133a、-206均能促进成肌细胞的分化，其中miR-206在肌营养不良的病变过程中可能发挥重要作用。Objective： To study the change of muscle-specific miRNAs （miR-1, -133a, -206） in myoblast proliferation and differentiation so as to explore the relationship between miRNAs and muscular dystrophy. Methods： Mice myoblast C2 C12 was cultured and its differentiation was induced. The expression of miR-1, -133a, -206 in the myoblast was detected by quantitative real-time PCR analysis during C2C12 proliferation and differentiation （1, 3 and 5 days）. Dystrophin protein expression in the skeletal muscle was tested by immunohistochemistry assay, miR-1, -133a and -206 were detected in the skeletal muscle specimen of dystrophin-delete muscular dystrophy （DMD） patients. Results： The expression levels of miR-1, -133a and -206 were low in the myoblast proliferation stage and high in differentiation stage. Following the myoblast differentiation, the expression levels of muscle specific miRNAs gradually increased significantly, among which miR-1 increased the most. Compared with the control group, the expression levels of miR-1, -133a and -206 were higher in 10 cases of DMD patients, miR-206 was significantly upregulated in DMD. Conclusion： miR-1, -133a and -206 could promote myoblast differentiation. MiR-206 may play an important role in the pathophysiological process of DMD.

关 键 词：MIRNA dystrophin肌营养不良 成肌细胞 

分 类 号：R746.2[医药卫生—神经病学与精神病学]