行为学训练对大鼠海马缺血后神经干细胞增殖的影响  

Effect of behavior training on neural stem cells proliferation in ischemia hippocampus of rats

作　　者：邢雪松[1] 吕威力[2] 曾亮[1] 王正东[1] 

机构地区：[1]沈阳医学院,解剖学教研室,沈阳110034 [2]沈阳医学院,病理学教研室,沈阳110034

出　　处：《解剖学杂志》2016年第6期660-663,共4页Chinese Journal of Anatomy

基　　金：辽宁省科技厅科学技术计划资助项目(2013020216)

摘　　要：目的：探讨穿梭箱训练对大鼠海马缺血后糖原合成酶激酶-3β（GSK-3β）表达及对神经干细胞增殖分化的影响机制。方法：采用大脑中动脉栓塞（MCAO）制作大鼠血管性痴呆模型,免疫组织化学检测穿梭箱训练后海马神经干细胞BrdU的表达，观察穿梭箱训练对血管性痴呆大鼠神经干细胞增殖分化的影响。检测缺血海马GSK3β的表达，观察穿梭箱训练对血管性痴呆大鼠GSK-3β的影响。结果：脑缺血再灌注第3天缺血海马神经元BrdU阳性细胞明显增多，第7天达高峰。GSK-3β反应产物脑缺血再灌注第7天较正常组增多，随缺血再灌注时间的进一步延长逐渐减少，第14天后持续低水平表达。穿梭箱训练后BrdU、GSK-3β蛋白的表达明显增加。结论：穿梭箱训练促进神经干细胞的增殖及缺血脑组织GSK-3β的表达，其作用可能由GSK-3β信号介导。Objective. To study the effect of behavior training on the expression of GSK-3β and hippocampal neurogenesis in a rat model of cerebral ischemia/reperfusion, and to explore the related molecular and behavior training mechanism. Methods： Behavior training was performed at day I after the ischemia/reperfusion. Middle cerebral artery occlusion （MCAO） was performed to establish the vascular dementia model. The expression of BrdU and GSK-3β in the hippocampus was detected with immtmohistochemical methods to explore the related neurogenesis and behavior training molecular mechanism. Results： BrdU positive cells in the hippocampus obviously increased at clay 3 and peaked at clay 7 of ischemia/reper/usion. GSK-3β reaction products increased gradually with the time of cerebral ischemia/reperfusion, reached the peak on day 7, and gradually decreased. After training, the expression of GSK-3β in hippocampous had apparently increased in each group. Conclusion： Behavior training promotes the expression of GSK-3β in the hippocampus tissues. These findings indicate that promotion of neural stem cell proliferation induced by behavior training may be mediated by GSK-3β signaling pathway.

关 键 词：行为学训练 糖原合成酶激酶-3B 神经干细胞 增殖 大脑中动脉栓塞 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]