转大豆泛素活化酶GmUBA1基因烟草的抗冷性研究  

Cold Tolerance Research of the GmUBA1 Gene in Transgenic Tobacco Plants

作　　者：杜秋丽[1] 赵强[1] 石会会 朱九滨[1] 胡彦营[1] 王晓强[1] 李红燕[1] DU Qiuli ZHAO Qiang SHI Huihui ZHU Jiubin HU Yanying WANG Xiaoqiang LI Hongyan(Department of Life Science and Engineering, Jining University, Qufu 273155,China)

机构地区：[1]济宁学院生命科学与工程系,山东曲阜273155

出　　处：《济宁学院学报》2016年第6期34-37,共4页Journal of Jining University

基　　金：山东省自然科学基金项目(ZR2012CM011);山东省高校科技计划项目(J11LC56);济宁市优秀中青年科研创新资金计划(2010JNKJ102);济宁学院教改项目(2012JX29)

摘　　要：研究大豆泛素活化酶基因对转基因烟草耐低温能力的影响.利用转Gm UBA1烟草的种子,通过比较低温处理后对照和转基因系烟草幼苗根长与湿重的生长状况,运用生物统计学方法分析Gm UBA1基因对烟草耐低温能力的调控效应.低温胁迫下,转反义泛素活化酶基因烟草苗的湿重及根长均明显高于对照组CK,转正义泛素活化酶基因烟草苗的湿重及根长均明显低于CK.转反义泛素活化酶基因可以提高转基因烟草植株的耐寒性,改善低温胁迫下植株的生理功能,进而提高植株抵御低温胁迫的能力.To elucidate the effects of GmUBA1 on low-temperature stress tolerant capacities in transgenic tobacco. After low-temperature treatment, the regulation effects of GmUBA1 gene were evaluated by the comparisons of wet weight and the length of roots among the anti-expression and over-expression of GmUBAltransgenic lines and control （CK）. By using biostatistics, GmUBA1 gene effects of transgenic tobacco＇ s low-temperature tolerant capacities were analyzed.Under low temperature stress, wet weight and the length of roots of anti-expression of GmUBA1 in transgenic tobacco plants were higher;wet weight and the length of roots of over-expression of GmUBA1 in transgenic tobacco plants were lower.These results suggest that anti-expression of GmUBA1 in transgenic tobacco could increase cold tolerance of transgenic tobacco plants, improve physical function of plants under low temperature stress, may further improve the ability of plants to withstand cold stress.

关 键 词：泛素蛋白酶体途径 E1泛素活化酶 转基因烟草 耐低温能力 

分 类 号：F590.3[经济管理—旅游管理]