肾病综合征患者外周血高迁移率族蛋白-1和NF-KB的表达水平及相关性研究  

The expression and correlation study of high mobility group-1 and NF-KB in Peripheral blood of Nephritic syndrome patients

作　　者：史学颖[1] 张菊香[2] 

机构地区：[1]内蒙古包钢医院肾内科,内蒙古包头014010 [2]内蒙古包钢医院心内科

出　　处：《疾病监测与控制》2016年第12期973-974,共2页Journal of Diseases Monitor and Control

摘　　要：目的研究肾病综合征(Nephritic syndrome,NS)患者外周血中高迁移率族蛋白-1(high mobility group-1,HMGB-1)和NF-KB对NS患者的临床作用和相关性影响。方法设定NS患者为观察组(n=137)和健康志愿者为对照组(n=53),同时采用进行酶联免疫吸附分析法(enzyme linked immunosorbent assay,ELISA)测定外周血中HMGB-1和NF-KB的数值,并且对检测结果与不同组患者的24小时尿蛋白和血清白蛋白指标进行相关性分析。结果 (1)外周血HMGB-1平均检测结果:观察组在(30.02±2.36)ng/l,其中重型在(34.20±3.56)ng/l,轻型在(25.46±0.56)ng/l;对照组平均检测结果在(9.83±1.17)ng/l;重型患者HMGB-1检测指标高于轻型患者(P﹤0.05)。观察组HMGB-1检测指标在重度、轻度NS患者中的水平均显著高于对照组的水平(P﹤0.05)。(2)外周血NF-KB水平检查结果:观察组在(1 303.75±49.12)ng/l,其中重型在(1 485.32±321.41)ng/l,轻型在(1 179.23±456.01)ng/l;对照组平均检测结果在(501.79±56.89)ng/l;重型患者NF-KB检测指标高于轻型患者(P﹤0.05)。观察组NF-KB检测指标在重度、轻度NS患者中的水平均显著高于对照组的水平(P﹤0.05)。(3)HMGB-1和NF-KB水平呈正相关性(R2=0.173,P﹤0.01)。结论 NS患者外周血中HMGB-1和NF-KB水平均高于健康人群水平,HMGB-1对NF-KB具有正性影响,两组检测结果对疾病的诊断和病情分型具有重要临床意义,为治疗提供了更加可靠的分子水平依据,值得推广。Objective To study the clinical effect and correlation of high mobility group-1 and NF-KB from peripheral blood of Nephritic syndrome（NS）patients. Methods 137 NS patients were defined as the observation group, and 53 healthy volunteers defined as the control group, ELISA method was used to determine the level of HMGB-1 and NF-KB in peripheral blood of both the observation group and the control group, correlation analysis was made between the detection results and 24-hour urinary protein and serum albumin index of cases from both groups. Results（1） The detection results of HMGB-1 in peripheral blood were： the average level of the observation group was（30.02±2.36）ng/l,（34.20±3.56）ng/l for the severe patients,（25.46±0.56）ng/l for the linght patients,the detection index of the severe patients was higher than the light ones, the average level of the control group was（9.83±1.17）ng/l. The HMGB-1 level of both the severe patients and the light patients of the observation group were significantly higher than the level of the control group（P 〈0.05）.（2） The detection results of NF-KB in peripheral blood were： the average level of the observation group was（1 303.75±49.12）ng/l,（1 485.32±321.41）ng/l for the severe patients,（1 179.23±456.01）ng/l for the light patients, the severe ones higher than the light ones, the average level of the control group was（501.79±56.89）ng/l. The NF-KB level of both the severe patients and the light patients of the observation group were significantly higher than the level of the control group（P〈0.05）.（3） The detection levels of HMGB-1 and NF-KB were positively correlated（R2=0.173, P 〈0.01）. Conclusion Both HMGB-1 and NF-KB level of NS patients were higher than those of healthy people, HMGB-1 has positive relation to NF-KB, the detection has clinical significance to the diagnosis and classification of the disease, and provide a more reliable molecular level basis for treatment, it is worth promoting.

关 键 词：肾病综合征 外周血 高迁移率族蛋白-1 NF-KB 

分 类 号：R692.5[医药卫生—泌尿科学]