短小芽孢杆菌表面活性素基因克隆及其抑菌活性研究  

Surfactin genes cloning and determination of its inhibition activity of Bacillus pumilus

作　　者：程杰杰[1] 孙帅欣 殷超凡 陈云鹏[1] 

机构地区：[1]上海交通大学农业与生物学院/农业部都市农业(南方)重点实验室,上海200240

出　　处：《中国植保导刊》2016年第12期5-9,共5页China Plant Protection

基　　金：国家自然科学基金面上项目(31071721)

摘　　要：以短小芽孢杆菌(Bacillus pumilus)DX01菌株为供试对象,以其基因组DNA为模板,通过PCR扩增,成功克隆出表面活性素基因。构建了原核表达载体pET28a-SURF1,在大肠杆菌BL21中进行了IPTG诱导表达,经过柱纯化和聚丙烯酰胺凝胶电泳(SDS-PAGE),证实pET28a-SURF1在大肠杆菌BL21中成功表达。通过玉米小斑病病菌分生孢子萌发试验、对玉米小斑病病和稻瘟病病菌平板抑菌试验,测定了表面活性素融合蛋白对玉米小斑病病菌和稻瘟病两种病菌的抑菌活性。Based on genomic DNA template of Bacillus pumilus DX01, prokaryotic expression vector pET28-Surf1 was con- strueted by surfactin genes clone with PCR amplification, which successfully expressed in E. coli BL21 with IPTG induction by column purification and SDS-PAGE. Growth inhibitory activity of fused proteins against Bipolaris maydis and Magnaporthe oryzae were determined by tests of Bipolaris maydis conidium germination and tablet inhibition of the two phytopathogens.

关 键 词：短小芽孢杆菌 表面活性素 原核表达 抑菌活性 

分 类 号：Q785[生物学—分子生物学] S435.131[农业科学—农业昆虫与害虫防治]