虾青素对糖尿病大鼠海马脑源性神经营养因子表达的影响  

Effect of astaxanthin on expression of brain-derived neurotrophic factor of diabetic encephalopathy in diabetic rats

作　　者：刘庆春[1] 

机构地区：[1]南阳医学高等专科学校基础医学部,河南473061

出　　处：《社区医学杂志》2016年第23期5-7,共3页Journal Of Community Medicine

基　　金：河南省重点科技攻关项目(122102310103)

摘　　要：目的探讨虾青素对糖尿病大鼠海马脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)表达的影响。方法腹腔注射链脲佐菌素建立糖尿病大鼠模型,造模成功后,分为模型组、阳性对照组和虾青素组。阳性对照组和虾青素组分别灌胃盐酸二甲双胍和虾青素溶液。正常组灌胃等量生理盐水。药物干预6周后,分别对各组大鼠进行Morris水迷宫实验,采用real-time PCR法和ELISA法分别检测海马BDNF的表达和含量。多个样本均数比较采用单因素方差分析,两两比较采用LSD法,P<0.05为差异有统计学意义。结果四组大鼠的平台象限路径百分比和平台象限停留时间[(0.615 7±0.042)、(0.321 5±0.020)、(0.533 6±0.027)、(0.498 6±0.029)%与(43.37±6.98)、(21.46±5.65)、(36.64±5.87)、(33.18±6.35)s]比较,差异均有统计学意义(均P<0.05)。两两比较发现,与正常组相比,模型组大鼠在平台象限路径百分比和平台象限停留时间均显著减少,差异均有统计学意义(均P<0.05)。与模型组相比,阳性对照组和虾青素组在平台象限路径百分比和平台象限停留时间明显提高,差异均有统计学意义(均P<0.05)。四组大鼠BDNF含量[(5.38±0.96)、(1.72±0.33)、(3.54±0.62)、(3.17±0.57)pg/ml]比较,差异有统计学意义(P<0.05),与正常组比较,模型组BDNF的含量降低了68.03%,差异有统计学意义(P<0.05)。四组大鼠BDNF m RNA相对表达量[(0.98±0.17)、(0.53±0.10)、(0.74±0.11)、(0.75±0.12)]比较,差异有统计学意义(P<0.05),模型组BDNF的m RNA水平与正常组相比降低了45.92%,差异有统计学意义(P<0.05)。结论虾青素具有提高糖尿病大鼠认知功能的作用,其机制可能与上调BDNF的表达有关。Objective To explore the effect of astaxanthin on expression of brain-derived neurotrophic factor（BDNF） in hippocampus in diabetic rats. Methods Rats were injected with streptozotocin to established diabetic model, then animals were divided into model group, positive control group and astaxanthin group in which those were treated with salt water, metformin and astaxanthin. After six weeks of drug treatment, rats were treated with morris water maze test, and hippocampus were sampled. The expression and content of hippocampal BDNF were detected by real-time PCR and ELISA. Measurement data was processed by variance analysis, pairwise comparison was used by LSD. Count data was used by chi square test, P〈0.05 was considered statistically significant. Results There were significant differences in the percentage of platform quadrant path and the time stayed in the plate quadrant[（0.615 7±0.042）,（0.321 5±0.020）,（0.533 6±0.027）,（0.498 6±0.029）% and（43.37±6.98）,（21.46±5.65）,（36.64±5.87）,（33.18±6.35）s] in the four groups（all P〈0.05）. Compared with the normal group, the percentage of platform quadrant path and the time stayed in the plate quadrant in the model group were significantly reduced, the differences were statistically significant（all P〈0.05）. Compared with the model group, the percentage of platform quadrant path and the time stayed in the plate quadrant in the positive control group and astaxanthin group were significantly increased, the differences were statistically significant（all P〈0.05）. There was a significant difference in BDNF [（5.38±0.96）,（1.72±0.33）,（3.54±0.62）,（3.17±0.57） pg/ml] in four groups（P〈0.05）. Compared with thenormal group, the level of BDNF in model group was decreased by 68.03%, the difference was statistically significant（P〈0.05）. There was a significant difference in relative expression of BDNF[（0.98±0.17）,（0.53±0.10）,（0.74±0.11）,（0.75±0.12）]（P〈0.05）. Compared with the n

关 键 词：虾青素 大鼠 糖尿病 脑源性神经营养因子 

分 类 号：R34[医药卫生—基础医学]