男性不育患者Y染色体微缺失和生殖激素的相关性研究  

Research on the correlation of Y chromosome microdeletions and reproductive hormones in male infertile patients

作　　者：程玲[1] 张玲[1] 蓝惠华[1] 王玮玮[1] 杨艳[1] 陈涌泉[1] 王厚照[1] CHENG Ling ZHANG Ling LAN Hui-hua WANG Wei-wei YANG Yan CHEN Yong-quan WANG Hou-zhao.(The Central Laboratory of the 174th Hospital of PLA, The Affiliated Cheng gong Hospital of Xiamen University, The 174th Clinical College of Anhui Medical University, Xiamen 361003)

机构地区：[1]中国人民解放军第一七四医院中心实验室,厦门大学附属成功医院,安徽医科大学解放军174临床学院,厦门361003

出　　处：《中国优生与遗传杂志》2016年第12期45-46,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨男性不育患者Y染色体微缺失的分布和生殖激素水平的变化,分析其相关性。方法选取2015年8月-2016年7月就诊于我院生殖医学中心的男性不育患者717例,抽取外周血清,采用荧光定量PCR方法检测Y染色体微缺失,化学发光仪检测生殖激素水平。同时选取80例正常生育男性为对照组。结果 717例男性不育患者中,共有27例出现Y染色体微缺失,缺失率为3.8%(27/717),其中AZFa区缺失1例,缺失率为0.14%(1/717),AZFb区缺失1例,缺失率为0.14%(1/717),AZFc区缺失23例,缺失率为3.2%(23/717),AZFb+c区缺失2例,缺失率为0.28%(2/717)。与无AZF缺失不育组和对照组比较,AZF缺失不育组FSH水平显著升高(P<0.05),E2、LH、PRL和T无明显差异(P>0.05)。结论 AZFc区缺失是男性不育患者Y染色体微缺失最常见的缺失类型,缺失位点为s Y254和s Y255;FSH水平增高与AZF缺失不育密切相关。Objective：To explore the diffusion of Y chromosome microdeletions and reproductive hormones in infertile patients,and analyse the relationships. Methods：717 patients who came to the reproductive medical center during 2015.08 to 2016.07 were selected,the fluorescent quantitation PCR was used to detect the Y chromosome microdeletions,the Chemiluminescence apparatus was used for the level of reproductive hormones.80 healthy fertile men were selected as control group. Results：There were 27 Y chromosome microdeletions patients of the 717 infertile patients,the microdeletion rate was 3.8%（27/717）,of the 27 cases,AZFa、AZFb、AZFc、AZFb＋c region were respectively 1 、1、23、2 cases,the microdeletion rates were respectively 0.14%（1/717）、0.14%（1/717）、3.2%（23/717）、0.28%（2/717）. Compared with the infertile group without AZF microdeletion and the control group,the level of FSH was significant higher in groups with AZF microdeletion（P〈0.05）,and there was no obvious difference in E2、LH、PRL and T. Conclusions：The AZFc region was the most common type of Y chromosome microdeletion,the site were s Y254 and s Y255;the increase of FSH was closely related with AZF deletion in infertile patients.

关 键 词：男性不育 Y染色体微缺失 生殖激素 

分 类 号：R698.2[医药卫生—泌尿科学]