Low expression of centrosomal protein 78(CEP78)is associated with poor prognosis of colorectal cancer patients  

作　　者：Meifang Zhang Tingmei Duan Li Wang Jianjun Tang Rongzhen Luo Ruhua Zhang Tiebang Kang 

机构地区：[1]State Key Laboratory of Oncology in South China,Collaborative Innova-tion Center for Cancer Medicine,Sun Yat-sen University Cancer Center,Guangzhou 510060,Guangdong,P.R.China. [2]Department of Pathology,Sun Yat-sen University Cancer Center,Guangzhou 510060,Guangdong,P.R.China. [3]Research Department,Sun Yat-sen University Cancer Center,Guang-zhou 510060,Guangdong,P.R.China.

出　　处：《Chinese Journal of Cancer》2016年第10期509-517,共9页

摘　　要：Background: Centrosomal protein 78(CEP78) has been characterized as a component of the centrosome required for the regulation of centrosome-related events during the cell cycle, but its role in human cancers remains unclear. This study aimed to investigate the role and the clinical value of CEP78 in colorectal cancer(CRC).Methods: Quantitative real-time polymerase chain reaction(q RT-PCR) and immunohistochemistry were performed to examine CEP78 expression in CRC tissues and adjacent noncancerous tissues. The association between CEP78 expression and clinical outcomes of CRC patients was determined. The effect of CEP78 on cell growth was examined in vitro by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay, colony formation, and flow cytometry assays and in vivo using a nude mouse model.Results: The expression level of CEP78 was significantly lower in tumor tissues than in the adjacent normal tissues(P < 0.01). Low CEP78 expression was significantly associated with poor differentiation(P etastasis(P = 0.003), large tumor size(P = 0.017), lymphatic mtient= 0.034), distant metastasis(P s with low CEP78 expression h= 0.029), and advanced stage(P Meier analysis indicated that paad shorter survival than those wit= 0.011). Kaplan–h high CEP78 expression(P < 0.01). Overexpression of CEP78 in CRC cells significantly reduced cell viability and colony formation in vitro and halted tumor growth in vivo. Further study showed that CEP78 reintroduction in CRC cells resulted in G2/M phase arrest rather than cell apoptosis.Conclusions: CEP78 might function as a tumor suppressor and serve as a novel prognostic marker in CRC.Background： Centrosomal protein 78（CEP78） has been characterized as a component of the centrosome required for the regulation of centrosome-related events during the cell cycle, but its role in human cancers remains unclear. This study aimed to investigate the role and the clinical value of CEP78 in colorectal cancer（CRC）.Methods： Quantitative real-time polymerase chain reaction（q RT-PCR） and immunohistochemistry were performed to examine CEP78 expression in CRC tissues and adjacent noncancerous tissues. The association between CEP78 expression and clinical outcomes of CRC patients was determined. The effect of CEP78 on cell growth was examined in vitro by 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide（MTT） assay, colony formation, and flow cytometry assays and in vivo using a nude mouse model.Results： The expression level of CEP78 was significantly lower in tumor tissues than in the adjacent normal tissues（P 〈 0.01）. Low CEP78 expression was significantly associated with poor differentiation（P etastasis（P = 0.003）, large tumor size（P = 0.017）, lymphatic mtient= 0.034）, distant metastasis（P s with low CEP78 expression h= 0.029）, and advanced stage（P Meier analysis indicated that paad shorter survival than those wit= 0.011）. Kaplan–h high CEP78 expression（P 〈 0.01）. Overexpression of CEP78 in CRC cells significantly reduced cell viability and colony formation in vitro and halted tumor growth in vivo. Further study showed that CEP78 reintroduction in CRC cells resulted in G2/M phase arrest rather than cell apoptosis.Conclusions： CEP78 might function as a tumor suppressor and serve as a novel prognostic marker in CRC.

关 键 词：中心体蛋白 结直肠癌 预后 患者 细胞周期 聚合酶链反应 RT-PCR 实验检测 

分 类 号：R735.34[医药卫生—肿瘤]