ITP中T细胞活化信号分子c-Cbl和Cbl-b的表达变化  

Change in expression pattern of T cell activation signal molecules c-Cbl and Cbl-b in peripheral blood of patients with ITP

作　　者：钟隽[1] 陈少华[2] 郁志[1] 陆帅[2] 谭家雄 杨力建[2] 李萡[2] 李扬秋[1,2,3] 

机构地区：[1]暨南大学附属第一医院血液科,广东广州510632 [2]暨南大学医学院血液病研究所,广东广州510632 [3]暨南大学再生医学教育部重点实验室,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2016年第6期472-475,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金项目(81370605;81570143)

摘　　要：目的:分析免疫性血小板减少症(ITP)中T细胞活化信号通路相关分子Cbl-b和c-Cbl基因的表达情况.方法:利用SYBR Green实时荧光定量聚合酶链式反应(RT-PCR)方法检测18例ITP患者外周血单个核细胞(PBMC)中Cbl-b和c-Cbl基因的表达水平,20例健康成人作为对照.结果:ITP组Cbl-b的表达水平(中位数为0.091%)与健康成人组Cbl-b的表达水平(中位数为0.366%)相比,明显降低(P<0.001);而c-Cbl基因在ITP组中的表达水平(中位数为0.372%)与健康成人组的表达水平(中位数为0.298%)相比没有统计学差异,但c-Cbl基因在ITP女性患者样本中的表达水平(中位数为0.236%)明显低于男性患者样本c-Cbl的表达水平(中位数为0.479%)(P=0.039).结论:ITP患者外周血中Cbl-b表达下调,可能是其T细胞免疫异常活化的原因之一.Aim： To analyze the expression level of T cell activation signal molecules c-Cbl and Cbl-b in peripheral blood of patients with Immune thrombocytopenic purpura. Methods SYBR Green PCR technique was used to detect expression level of c-Cbl and Cbl-b genes in peripheral blood mononuclear cells （PBMCs） from 18 patients with ITP, 20 healthy individuals were served as control. Results： The significant lower expression level of Cbl-b （median; 0 .0 9 1% ） was found in ITP group in comparison with healthy group （ median ; 0. 366% , P 〈 0. 001） . Although there was not significant different on the c-Cbl expression level between ITP group （median; 0. 372% ） and healthy group （median; 0. 298% ） , interesting, the significant lower expression level of c-Cbl （median; 0 .2 3 6% ） was found in female pa-tients in comparison with male patients （median; 0. 479% , P = 0 .0 3 9 ） with ITP. Conclusion ; The down regulation of Cbl-b might be one of the reasons of abnormal T cell immune activation in peripheral blood of patients with ITP.

关 键 词：免疫性血小板减少症 CBL-B C-CBL 基因表达 

分 类 号：R558.2[医药卫生—血液循环系统疾病] R392.12[医药卫生—内科学]