Clostridium acetobutylicum的亚硝基胍和钴-60复合诱变  

COMPOUND MUTATION OF Clostridium acetobutylicum WITH NITROSOGUANIDINE AND COBALT-60

作　　者：冯鸿燕[1,2] 李东[1,3] 闫志英[1] 袁月祥[1] 傅舒[1,2] 刘晓风[1] 廖银章[1] Feng Hongyan Li Dong Yan Zhiying Yuan Yuexiang Fu Shu Liu Xiaofeng Liao Yinzhang(Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China University of Chinese Academy of Sciences, Beijing 100049, China Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China)

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]中国科学院大学,北京100049 [3]中国科学院可再生能源重点实验室,广州510640

出　　处：《太阳能学报》2016年第12期3240-3245,共6页Acta Energiae Solaris Sinica

基　　金：中国科学院可再生能源重点实验室(y407k11001)

摘　　要：利用化学诱变剂亚硝基胍及物理诱变剂钴-60对丙酮丁醇梭菌YBD(Clostridium acetobutylicum YBD)进行复合诱变,结合多次传代培养,筛选获得一株编号为500-1的突变株。该菌株经6代培养后丁醇产量为15.40 g/L;且能耐受15 g/L的丁醇;在10%玉米醪培养基中,丁醇产量为15.57 g/L,较出发菌株提高27.62%;在10 L发酵罐发酵实验中,以玉米粉为发酵原料,36 h时突变株丁醇产量可达14.24 g/L,生产速率为0.40 g/(L·h),较出发菌株的生产速率提高207.69%。Clostridium acetobutylicum YBD was mutated by chemical mutagens nitrosoguanidine （NTG） and physical mutagens cobalt-60, and a mutant strain, recorded 500-1 with multiple generations culture was acquired. After six generations culture, the mutation strain could produce butanol of 15.40 g/L and tolerate butanol of 15 g/L. The mutant strain could produce the butanol quantity reached 15.57 g/L. Compared with the original strain, the butanol quantity increased by 27.62%, respectively. In the 10 L-fermentation experiment with corn flour as the raw material, the butanol quantity of mutant strain of 14.24 g/L was achieved after 36 hours, and the yield was 0.40 g/（L·h） which was increased by 207.69% than the yield of initial strain.

关 键 词：丙酮丁醇梭菌 复合诱变 丙酮-丁醇-乙醇发酵 稳定性 丁醇耐受性 

分 类 号：TQ923[轻工技术与工程—发酵工程]