Comparison of time-domain,spectral-domain and swept-source OCT in evaluating aqueous cells in vitro  

作　　者：Meng Li Huaizhou Wang Yue Liu Xifang Zhang Ningli Wang 

机构地区：[1]Beijing Institute of Ophthalmology,Beijing Tongren Eye Center,Beijing Tongren Hospital,Capital Medical University,Beijing Ophthalmology&Visual Sciences Key Laboratory,Beijing 100005,China [2]Department of Ophthalmology,The affiliated hospital of Guizhou Medical University,Guiyang 550001,China

出　　处：《Science China(Life Sciences)》2016年第12期1319-1323,共5页中国科学（生命科学英文版）

基　　金：supported by Beijing Scholar Program

摘　　要：Different optical coherence tomography （OCT）, both tirne-domain OCT and spectral-domain OCT, has been used to evaluate anterior chamber cells in previous studies. Recently, commercial swept-source OCT was available. The comparison among three kinds of OCTs have not been done before. Here, we compared their feasibility in discerning aqueous cells in vitro. The peripheral blood was diluted to eight different cell concentrations and was packed into plastic-film bag. Each sample was test- ed using different scan modes of three OCTs. It was easy to discern aqueous cells from background noise in both Visante AS-OCT and RTVue XR Avanti OCT, but difficult in Casia SS-1000 OCT. The measured blood cell densities in high-resolution corneal cross-sectional scan of Visante AS-OCT, cornea line scan and 3D cornea scan of RTVue XR Avanti OCT were highly correlated with the actual blood cell concentration （Pearson correlation=0.991, 0.989, and 0.993 respective- ly）. The mean adjusted cell densities of cornea line scan （10.46±2.87 spots mm-2） and 3D cornea scan （11.01±2.47 spots mm-2） of RTVue XR Avanti OCT were significantly larger than that of Visante AS-OCT （1.55±1.75 spots mm-2） （P〈0.01）. Our study demonstrated that RTVue XR Avanti OCT, a spectral-domain OCT, is superior to other two OCTs in evaluating aqueous cells.Different optical coherence tomography(OCT), both time-domain OCT and spectral-domain OCT, has been used to evaluate anterior chamber cells in previous studies. Recently, commercial swept-source OCT was available. The comparison among three kinds of OCTs have not been done before. Here, we compared their feasibility in discerning aqueous cells in vitro. The peripheral blood was diluted to eight different cell concentrations and was packed into plastic-film bag. Each sample was tested using different scan modes of three OCTs. It was easy to discern aqueous cells from background noise in both Visante AS-OCT and RTVue XR Avanti OCT, but difficult in Casia SS-1000 OCT. The measured blood cell densities in high-resolution corneal cross-sectional scan of Visante AS-OCT, cornea line scan and 3D cornea scan of RTVue XR Avanti OCT were highly correlated with the actual blood cell concentration(Pearson correlation=0.991, 0.989, and 0.993 respectively). The mean adjusted cell densities of cornea line scan(10.46±2.87 spots mm^(-2)) and 3D cornea scan(11.01±2.47 spots mm^(-2)) of RTVue XR Avanti OCT were significantly larger than that of Visante AS-OCT(1.55±1.75 spots mm^(-2))(P<0.01). Our study demonstrated that RTVue XR Avanti OCT, a spectral-domain OCT, is superior to other two OCTs in evaluating aqueous cells.

关 键 词：optical coherence tomography UVEITIS aqueous cell 

分 类 号：R773[医药卫生—眼科]