手足综合征大鼠模型血浆细胞因子的变化  

The Change on Cytokines in Rat Plasma of HFS

作　　者：黎鹏[1] 王炳胜[2] 李永民[3] LI Peng WANG Bing-sheng LI Yong-min(Graduate School of Hebei North University, Zhangjiakou 075000, China PLA 251 Hospital, Zhangfiakou 075000, China Hebei North University,Zhangjiakou 075000, China)

机构地区：[1]河北北方学院研究生院,河北张家口075000 [2]解放军第251医院,河北张家口075000 [3]河北北方学院,河北张家口075000

出　　处：《中国皮肤性病学杂志》2017年第1期24-26,30,共4页The Chinese Journal of Dermatovenereology

基　　金：全军"十二五"中医药重点项目(10ZYZ105);河北省研究生创新资助项目(285)

摘　　要：目的建立大鼠手足综合征模型,探讨手足综合征发病过程中相关炎性因子的水平及发病机制。方法运用卡培他滨制作手足综合征大鼠模型,分为给药1周期的A组和给药两周期的B组(连续给药7d,间隔3d,10d为一个周期),分别模拟不同给药剂量下发生的手足综合征,留取大鼠足跖部图像资料及血浆标本,检测血浆中亲胆碱能神经元因子(CNTF)、血小板衍生的内皮细胞生长因子(PD-ECGF)、白介素β(IL-β)、白介素10(IL-10)、中性粒细胞趋化因子(CINC-3)水平。结果与正常大鼠相比,1周期模型大鼠PD-ECGF,CINC-3,CNTF,IL-10,IL-β水平均升高(P<0.01);而1周期造模未成功大鼠、模型大鼠停药1周后各细胞因子水平与正常大鼠相比的差异无统计学意义(P>0.05)。与正常大鼠相比,2周期模型大鼠及模型大鼠停药1周后CINC-3,CNTF水平明显升高(P<0.01);而余各因子在各组间水平差异无统计学意义(P>0.05)。结论在短期给药过程中,HFS的发生主要与广泛发生的炎性损伤有关,而在较长时间的给药后,广泛的炎性反应趋于控制,而主要反应为相关炎性细胞的趋化及神经元的损伤。Objective To establish a rat model of hand-foot syndrome and investigate the expression of cytokines and the pathogenesis of hand foot syndrome. Methods To built the animal models of HFS with capecitabine that were divided into two groups （A for 1 cycle and B for 2 cycles） with different dosage（ continuous administration for 7 days, interval of 3 days, 10 days for a cycle） , then reversing image data of rat foot plantar and serum samples, the expression of PD-ECGF, CINC-3, CNTF,IL-10,IL-β were determined. Results To compare with the normal rats, the expression of PD-ECGF, CINC-3, CNTF, IL-10, IL-β increased in the animal models with 1 cycles of administration（ P 〈 0.01 ） ,but there were no statistical difference in unsuccessful rat models with 1 cycles of administration and rat models with stopping medicine for 1 week （ P 〉 0. 05 ）. To compare with the normal rats, the expression of CINC-3 and CNTF increased in the animal models with 2 cycles of ad- ministration and the rat models with stopping medicine for 1 week（ P 〈 0. 01 ）, and there was no significant difference in the expression of other factors between the different groups （ P 〉 0. 05 ）. Conclusion HFS is mainly related to the occurrence of inflammatory injury, but after a long time of administration, the wide range of inflammatory responses tend to be controlled, and the related iifflammatory cell chemotaxis and neuronal damage are dominant.

关 键 词：卡培他滨 手足综合征 发病机制 动物模型 

分 类 号：R730.5[医药卫生—肿瘤]