西奥骨化醇抑制TGF-β1诱导的肝癌细胞SMMC-7721上皮-间质转化  

Seocalcitol inhibits transforming growth factor-β1-induced epithelial-mesenchymal transition in hepatoma carcinoma SMMC-7721 cells

作　　者：曹令平 涂利宽[1] 吴小翎[1] Cao Lingping Tu Likuan Wu Xiaoling(Department of Gastroenterology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China)

机构地区：[1]重庆医科大学附属第二医院消化内科,重庆400010

出　　处：《第三军医大学学报》2017年第1期42-47,共6页Journal of Third Military Medical University

摘　　要：目的研究西奥骨化醇(seocalcitol,EB1089)对TGF-β1诱导肝癌细胞SMMC-7721上皮-间质转化(epithelial-mesenchymal transitions,EMT)的抑制作用。方法用不同浓度维生素D类似物EB1089(1、10、100、1 000 nmol/L)作用于肝癌细胞SMMC-7721 6、12、24、48 h,筛选出具有统计学意义的作用浓度和时间。将肝癌细胞SMMC-7721分为4组(EB1089组、TGF-β1组、EB1089+TGF-β1组和空白对照组),分别干预48 h。用相差倒置显微镜观察细胞形态变化;通过实时RT-PCR和Western blot分别检测E-cadherin、N-cadherin、Vimentin的mRNA和蛋白表达情况;迁移实验和侵袭实验检测EB1089对肝癌细胞SMMC-7721侵袭和迁移能力的影响。结果 TGF-β1作用后肝癌细胞SMMC-7721呈现长梭形,并伸出较多触角,EB1089能明显改善这种形态变化;EB1089可有效升高TGF-β1所致的E-cadherin mRNA和蛋白低表达(P<0.05),同时降低N-cadherin、Vimentin mRNA和蛋白表达(P<0.05);EB1089对TGF-β1诱导的细胞侵袭、迁移具有抑制作用(P<0.05)。结论维生素D类似物EB1089能有效抑制TGF-β1诱导的肝癌细胞SMMC-7721上皮-间质转化现象和侵袭、迁移能力。Objective To determine the inhibitory effect of seocalcitol （EB1089） on transforming growth factorβ1 （TGF-β1）induced epithelial-mesenchymal transition （EMT） in the hepatoma carcinoma cell line SMMC-7721. Methods Vitamin D analog EB1089 at different concentrations （1, 10, 100 and 1 000 nmol/L） was used to treat the SMMC-7721 cells for different times （6, 12, 24 and 48 h） to determine the optimal dose and time. Then the SMMC-7721 cells were divided into control group, TGF-β1 group, EB1089 with or without TGF-β1 groups. Cell morphology was observed with phase-contrast microscopy. RT-PCR and Western blotting were employed to measure the expression of E-cadherin, N-caherin and Vimentin at mRNA and protein levels. Cell invasion and migration were evaluated by Transwell chamber assay. Results Compared with control cells, TGF-β1 stimulated cells developed an obvious long fusiform shape. Such morphological changes were prevented by the addition of EB1089. Meanwhile, EB1089 enhanced the protein and mRNA expression of E-cadherin suppressed by TGF-β1 （P〈0.05）, decreased the levels of N-caherin and Vimentin （P〈0.05）, and inhibited TGF-β1-induced invasiveness and migration in the SMMC-7721 cells. Conclusion Vitamin D analog EB1089 inhibits TGF-β1-induced EMT, and invasion and migration in the hepatoma carcinoma SMMC-7721 cells.

关 键 词：EB1089 上皮一间质转化 转化生长因子β1 肝癌 

分 类 号：R730.23[医药卫生—肿瘤] R735.7[医药卫生—临床医学]