槲寄生抗肿瘤有效成份提取及其对乳腺癌细胞的抑制作用  

Effective anti-tumor component separation of Viscum coloratum(kom) Nakai and its antitumor activity in breast cancer cells

作　　者：夏超[1] 郑马庆[2] 刘媛[2] 陈群英[1] 陈妍[3] 黄新恩[3] 许潇月 沈波[3] Xia Chao Zheng Maqing Liu Yuan Chen Qunying Chen Yan Huang Xin＇en Xu Xiaoyue Shen Bo(Jiangsu Provincial Institute of Materia Media,Nanjing Tech University,Nanjing 210009 School of Pharmaceutical Sciences ,Nanjing Tech University, Nanjing 211816 Department of Internal Medicine, Jiangsu Cancer Hospital Affiliated to NJMU,Nanjing 210009, China)

机构地区：[1]南京工业大学江苏省药物研究所有限公司,江苏南京210009 [2]南京工业大学药学院,江苏南京211816 [3]南京医科大学附属肿瘤医院内科,江苏南京210009

出　　处：《南京医科大学学报（自然科学版）》2016年第11期1313-1315,1396,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省卫生厅中医药管理局基金资助(LZ13232);吴阶平基金资助(320.6750.14193)

摘　　要：目的 :从槲寄生中分离、提取抗肿瘤有效组份,并观察该组份对乳腺癌细胞生长的影响。方法 :采用经典提取分离方法,利用槲寄生生物碱不同酸碱度在不同溶剂中溶解度不同,分离提取槲寄生不同组份;将槲寄生不同组份溶解并制成系列浓度的溶液后,加入到乳腺癌细胞中,观察该系列组份对乳腺癌细胞生长的影响。结果:采用经典提取分离法得到5个组份,利用生物碱不同酸碱度在不同溶剂中溶解性不同分离并纯化后,得到5个组份。这10个组份体外对乳腺癌细胞生长均有不同的抑制作用,且组份5能显著抑制乳腺癌细胞生长,对乳腺癌细胞系MDA-MB-231以及MDA-MB-435的IC50分别为3.24、4.80μg/m L。结论:槲寄生系列提取组份在体外对乳腺癌细胞的增殖具有不同的抑制作用,且组份5作用最强。Objective： To separate and extract effective components from Viscum coloratum（Kom.） Nakai and investigate their effects on the proliferation of breast cancer cells. Methods： The classical separation and extraction methods were used firstly,and then according to different solubilities of alkaloids from Viscum coloratum（Kom.） Nakai in various solvents with different p Hs,different components were extracted and obtained. The components were dissolved and cultured with breast cancer cells in vitro.Results： About 10 components were obtained and different inhibition effects were observed in co-culture with breast cancer cells.Component 5 inhibited the proliferation of breast cancer cells significantly in vitro and the IC50 for breast cancer cell line MDA-MB-435 and MDA-MB-231 was 3.24 mg/m L and 4.80 μg/ml,respectively. Conclusion：In vitro,series of extracts from Viscum coloratum（Kom） Nakai inhibited on breast cancer cells proliferation,and component 5 had significant inhibition effects on the cancer cells.

关 键 词：槲寄生 乳腺细胞系 IC50 

分 类 号：R737.9[医药卫生—肿瘤]