产麻痹性贝毒塔玛亚历山大藻共培养菌群的物种多样性研究  

Species diversity study of co-cultured bacterial community of toxic marine dnoflagellate Alexandrium tamarense

作　　者：刘巧红[1,2] 苑丽东 田晓清[1] 马丽艳[1] 周宏农[3] 樊成奇[1] 杨宪时[1] 杨桥[1,2,4] 张晓玲[1] LIU Qiao-hong YUAN Li-dong TIAN Xiao-qingI MA Li-yan ZHOU Hong-nong FAN Cheng-qi YANG Xian-shi YANG Qiao ZHANG Xiao-ling(East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China Shanghai Ocean University, Shanghai 201306, China Institute of Fisheries Science, National Taiwan University, Taipei 10617, China Department of Microbiology, Oregon State University, Corvallis 97333, OR, USA)

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090 [2]上海海洋大学,上海201306 [3]台湾大学渔业科学研究所,中国台北10617 [4]俄勒冈州立大学微生物学系,科瓦里斯俄勒冈97333

出　　处：《海洋渔业》2016年第6期663-669,共7页Marine Fisheries

基　　金：国家自然科学基金(41206093);中央公益性科研院所基本科研业务费专项(2012T08)

摘　　要：产毒赤潮藻与其共生菌的互作关系是揭示麻痹性贝毒(PSP)产生机制的关键,而其共培养菌群的分离及其多样性解析是开展此研究的必要前提。塔玛亚历山大藻(Alexandrium tamarense)是典型产PSP赤潮原因甲藻,为揭示其共培养菌群的物种多样性信息,本文通过免培养海洋微生物高通量测序技术,解析了东海塔玛亚历山大藻(at3#)共培养菌群的种类、丰度及多样性信息。结果表明,其共培养菌群共有87个种与已有数据库匹配,此外尚有5%的未知种。其包含57个属,其中优势菌属5个,分别为Rhodobacteraceae、Marinobacter、Methylophaga、Nitratireductor及Phycisphaera。研究表明东海产PSP塔玛亚历山大藻(at3#)含有物种丰富的共培养菌群,且具有发掘海洋特殊生境微生物新种属的良好潜力。Paralytic shellfish poisoning （PSP） is one of the four recognized syndromes of shellfish poisoning, which share some common features and are primarily associated with bivalve mollusks. These shellfish are filter feeders and, therefore, accumulate neurotoxins, called saxitoxin, produced by microscopic algae, such as dinoflagellates, diatoms and cyanobacteria. Dinoflagellates of the genus Alexandrium are the most numerous and widespread saxitoxin producers and are responsible for PSP blooms in subarctic, temperate and tropical areas. The majority of toxic blooms have been caused by the morphospecies Alexandrium catenella, Alexandrium tamarense and Alexandrium fundyense, which together comprise the A. tamarense species complex. PSP toxins produced by harmful algal blooms （HABs） can cause immense damage in many aspects. The answers to the source of PSP toxins are still unsolved. Algae-bacteria interaction study is the key to uncover the producing mechanism of PSP. Whereas, the intensive isolation and biological background analysis is the first premise to develop such interaction study. Currently, A. tamarense is a dinoflagellate known to produce saxitoxin, a neurotoxin which causes the human illness clinically known as PSP. Multiple species of phytoplankton are known to produce saxitoxin, including at least 10 other species from the genus Alexandrium. A. tamarense is a worldwide dinoflagellate known to produce saxitoxin. It has strong adaptabity, can survive in a wide range of sea areas. It distributes from Kiaochow Bay to Xiamen sea area and Dapeng Bay. It has frequency of HAB. A. tamarense is one of the representative toxic marine dinoflagellates causing HAB in the East China Sea area. However, little is known about its biodiversity of its co-cultured bacterial community yet. To elucidate its biodiversity, the species abundance and diversity of the co-cultured bacterial community associated with toxic A. tamarense（at3#） were analyzed by high-throughput sequencing. From the results, the quality control d

关 键 词：塔玛亚历山大藻 麻痹性贝毒 共培养菌群 物种多样性 

分 类 号：Q949.3[生物学—植物学]